| Semisynthesis of homogeneous spike RBD glycoforms from SARS-CoV-2 for profiling the correlations between glycan composition and function |
| Ye, FR; Li, C; Liu, FL; Liu, XL; Xu, P; Luo, RH; Song, WP; Zheng, YT; Ying, TL; Yu, B; Wang, P
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| 2024
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| 发表期刊 | NATIONAL SCIENCE REVIEW
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| ISSN | 2095-5138
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| 卷号 | 11期号:2 |
| 摘要 | Vaccines have been the primary remedy in the global fight against coronavirus disease 2019 (COVID-19). The receptor-binding domain (RBD) of the spike protein, a critical viral immunogen, is affected by the heterogeneity of its glycan structures and relatively low immunogenicity. Here, we describe a scalable synthetic platform that enables the precise synthesis of homogeneously glycosylated RBD, facilitating the elucidation of carbohydrate structure-function relationships. Five homogeneously glycosylated RBDs bearing biantennary glycans were prepared, three of which were conjugated to T-helper epitope (Tpep) from tetanus toxoid to improve their weak immune response. Relative to natural HEK293-derived RBD, synthetic RBDs with biantennary N-glycan elicited a higher level of neutralising antibodies against SARS-CoV-2 in mice. Furthermore, RBDs containing Tpep elicited significant immune responses in transgenic mice expressing human angiotensin-converting enzyme 2. Our collective data suggest that trimming the N-glycans and Tpep conjugation of RBD could potentially serve as an effective strategy for developing subunit vaccines providing efficient protection. Highly efficient semisynthetic procedure of homogeneously glycosylated RBDs was developed and further illuminated removal of sialic acids and trimming of N-glycans enhancing in vivo activity against SARS-CoV-2. |
| 收录类别 | sci
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/14208
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| 专题 | 科研部门_分子免疫药理学（郑永唐）
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推荐引用方式
GB/T 7714 |
Ye, FR,Li, C,Liu, FL,et al. Semisynthesis of homogeneous spike RBD glycoforms from SARS-CoV-2 for profiling the correlations between glycan composition and function[J]. NATIONAL SCIENCE REVIEW,2024,11(2).
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| APA |
Ye, FR.,Li, C.,Liu, FL.,Liu, XL.,Xu, P.,...&Wang, P.(2024).Semisynthesis of homogeneous spike RBD glycoforms from SARS-CoV-2 for profiling the correlations between glycan composition and function.NATIONAL SCIENCE REVIEW,11(2).
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| MLA |
Ye, FR,et al."Semisynthesis of homogeneous spike RBD glycoforms from SARS-CoV-2 for profiling the correlations between glycan composition and function".NATIONAL SCIENCE REVIEW 11.2(2024).
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