Evolutionarily distinct and sperm-specific supersized chromatin loops are marked by Helitron transposons in Xenopus tropicalis

	Evolutionarily distinct and sperm-specific supersized chromatin loops are marked by Helitron transposons in Xenopus tropicalis
	Shi, ZY; Xu, JS; Niu, LJ; Shen, W; Yan, ST; Tan, YJ; Quan, XB; Cheung, E; Huang, K; Chen, YL; Li, L; Hou, CH
	2023
发表期刊	CELL REPORTS
ISSN	2211-1247
卷号	42 期号:3
摘要	Transposable elements (TEs) are abundant in metazoan genomes and have multifaceted effects on host fitness. However, the mechanisms underlying the functions of TEs are still not fully understood. Here, we combine Hi-C, ATAC-seq, and ChIP-seq assays to report the existence of multimegabase supersized loop (SSL) clusters in the Xenopus tropicalis sperm. We show that SSL anchors are inaccessible and devoid of the architectural protein CTCF, RNA polymerase II, and modified histones. Nearly all SSL anchors are marked by Helitrons, a class II DNA transposon. Molecular dynamics simulations indicate that SSL clusters are likely formed via a molecular agent-mediated chromatin condensation process. However, only slightly more SSL anchor-associated genes are expressed at late embryo development stages, suggesting that SSL anchors might only function in sperm. Our work shows an evolutionarily distinct and sperm-specific genome structure marked by a subset of Helitrons, whose establishment and function remain to be explored.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14361
专题	科研部门_进化发育与四维基因调控（候春晖）
推荐引用方式 GB/T 7714	Shi, ZY,Xu, JS,Niu, LJ,et al. Evolutionarily distinct and sperm-specific supersized chromatin loops are marked by Helitron transposons in Xenopus tropicalis[J]. CELL REPORTS,2023,42(3).
APA	Shi, ZY.,Xu, JS.,Niu, LJ.,Shen, W.,Yan, ST.,...&Hou, CH.(2023).Evolutionarily distinct and sperm-specific supersized chromatin loops are marked by Helitron transposons in Xenopus tropicalis.CELL REPORTS,42(3).
MLA	Shi, ZY,et al."Evolutionarily distinct and sperm-specific supersized chromatin loops are marked by Helitron transposons in Xenopus tropicalis".CELL REPORTS 42.3(2023).

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