大豆是一种重要的经济作物。我与遗传发育做田志喜研究员研究组合作，对全世界的大豆品种做了全基因组变异分析。这项工作根据大豆驯化历程，从世界范围内选用了302个处于驯化不同阶段的代表性大豆种质为研究材料，进行重测序，与已经测序的大豆参考序列比较，得到群体SNP（单核苷酸突变）的信息。在分析群体结构后，我们依据人工选择的不同阶段和地理分布，将302个大豆划分为8个群体。通过农家种与野生种基因组分析，找到了121个驯化过程中受选择区域；通过改良品种与农家种的基因组分析，发现了109个改良相关的受选择区域。结合全基因组关联分析（GWAS）的数据，我们发现这些区域与重要农艺性状相关。另外我们对地理分布群体进行了群体基因组比较研究，找到一些与适应地理环境相关基因。本项工作已于2015时间发表Nature Biotechnology，本人作为共同贡献一作之一上，主要负责了大豆重测序和群体SNPs数据分析的工作。; Soybean (Glycine max) is an important crop. Collabarating with Prof. Zhixi Tian’s lab, which is a research group in the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, I took part in a comprehensive population genomics study on soybean. In this study, through a large-scale collection of soybean during domestication and improvement, we resequenced 302 wild, landrace and improved soybean accessions. Based on the soybean reference genome, 9,790,744 whole-genome SNPs were identified. We explored the phylogenetic relationships among the 302 accessions through whole-genome SNP analysis, and classified 302 accessions into several 8 clades, which exhibited strong geographic distribution patterns. By identifying potential selective signals during soybean domestication and improvement, we detected a total of 121 domestication-selective sweeps (landraces against wild soybeans) and 109 improvement-selective sweep (improved cultivars against landraces). Moreover, comparing genomics among sub-clades showed some traits and loci are associated with particular geographical regions. In this study, I mainly accomplished the genome sequencing and SNPs analysis.
修改评论