| A new lectin from the sea worm Serpula vermicularis: Isolation, characterization and anti-HIV activity | |
| Molchanova V*1; Chikalovets I1; Wang JH3; Yang DYO3; Zheng YT3; Lukyanov P1; Chernikov O1; Belogortseva N1; Li W2; molchanova@piboc.dvo.ru | |
| 2007 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
 |
| 卷号 | 145期号:2页码:184-193 |
| 合作性质 | 其它 |
| 摘要 | A GlcNAc-specific lectin was isolated from the sea worm Serpula vermicularis (SVL) (Annelida) and purified by ion-exchange, affinity and gel permeation chromatography. SVL was a homotetrameric protein with native molecular mass of about 50 kDa, and consisted of identical subunits of 12.7 kDa. The carbohydrate content of 1.9% suggested that the lectin was a glycoprotem, and mainly composed by aspartic and glutamic acids, glycine, valine and serine; with relatively lower content of basic amino acids and cysteine. The first 15 residues of the N-terminal region were determined as ADTPCQMLGSRYGWR. It was stable at pH 6-9 and at temperatures up to 40 degrees C. SVL was Ca2+-independent lectin that agglutinated native and trypsinized human erythrocytes. Hapten inhibition studies indicated that SVL showed binding specificity only for Nacetyl-D-glucosamine and its derivatives among the monosaccharides tested and required the presence of hydroxyl group at the C-3 of GlcNAc. The presence of hydrophobic p-nitrophenyl aglycone improved inhibitory potency of N-acetyl-D-glUCOsamine. Ovomucoid and ovalburnin were found to be inhibitors among the glycoproteins used for inhibition assay. The anti-HIV-1 (human immunodeficiency virus) activity of SVL in vitro was determined: SVL inhibited the production of viral p24 antigen and cytopathic effect induced by HIV-1. The EC50 values were 0.23 and 0.15 mu g-mL(-1) respectively. |
| 关键词 | Hiv-1 Antiviral Activity Glcnac-specific Lectin Marine Invertebrate Sea Worm Serpula Vermicularis |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/10538 |
| 专题 | 科研部门_分子免疫药理学（郑永唐） |
| 通讯作者 | molchanova@piboc.dvo.ru |
| 作者单位 | 1.Laboratory of Chemistry of Non-Infectious Immunity of Pacific Institute of Bioorganic Chemistry Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia 2.Key and Open Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, Department of Food Engineering, Dalian Fisheries University, Dalian 116023, China 3.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China |
| 推荐引用方式 GB/T 7714 | Molchanova V*,Chikalovets I,Wang JH,et al. A new lectin from the sea worm Serpula vermicularis: Isolation, characterization and anti-HIV activity[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2007,145(2):184-193. |
| APA | Molchanova V*.,Chikalovets I.,Wang JH.,Yang DYO.,Zheng YT.,...&molchanova@piboc.dvo.ru.(2007).A new lectin from the sea worm Serpula vermicularis: Isolation, characterization and anti-HIV activity.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,145(2),184-193. |
| MLA | Molchanova V*,et al."A new lectin from the sea worm Serpula vermicularis: Isolation, characterization and anti-HIV activity".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 145.2(2007):184-193. |
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| 2007145184.pdf（1059KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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