丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响

	丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响
其他题名	The effects of propofol on excitatory synaptic transmission in hippocampal CA1 neurons of rats
	谢玉波 1; 刘敬臣 1; 徐林*2; 曾邦雄 3
	2005
发表期刊	临床麻醉学杂志
卷号	21 期号:6 页码:399-401
合作性质	其它
摘要	目的观察500μmol/L丙泊酚对大鼠海马CA1区电刺激诱发的兴奋性突触后电流(EPSC)的影响,分析丙泊酚的可能作用机制。方法断头法分离Wistar大鼠(13~19d)海马半脑,用切片机切出400μm厚度的海马脑片,全细胞膜片钳技术记录CA1区锥体神经元EPSC。实验分两组:脂肪乳剂组(n=6)和丙泊酚组(n=10)。先以50μmol/L印防己毒素预孵脑片30min后,记录基础EPSC10min,然后加入450μl脂肪乳剂或丙泊酚(相当于500μmol/L),继续记录EPSC40min;继而以配对刺激代替单刺激,观察EPSC2/EPSC1比率的变化;改变膜钳制电压(-80~+60mV),观察电流-电压(I-V)曲线的变化。结果脂肪乳剂对EPSC无影响,500μmol/L丙泊酚降低大鼠海马CA1区EPSC值,25~30min左右达最大抑制效果,EPSC幅值下降至基础值的67·5%,明显低于脂肪乳剂组(P<0·05);而且500μmol/L丙泊酚明显降低EPSC2/EPSC1比率,也使I-V曲线左移,降低反转电位至-35mV左右。结论500μmol/L丙泊酚对大鼠海马CA1区兴奋性突触传递产生抑制作用,这可能与其增强突触前膜、突触后膜GABAA受体活性有关。; Objective 　 To investigate the effects of 500 μ mol/ L propofol on the whole 2 cell excit 2 atory postsynaptic current(EPSC) in hippocampal CA1 pyramidal neurons of rats and to analyze the mechanisms of propofol deeply. Methods 　 Wistar rats(13 2 19 d) were killed by cervical dislocation and hippocampal slices (400 μ m) were prepared. EPSCs were recorded by whole 2 cell patch 2 clamp tech 2 nique. The experiments were divided into two groups: the intralipid group ( n = 6) and the propofol group( n = 10) . After hippocampal slices were preincubated with 50 μ mol/L picrotoxin for 30 min ,the base value was recorded for 10 min and then 450 μ l intralipid or propofol(500 μ mol/L) was applied in perfusion for another 40 min. The Schaffer collateral/ commissural pathway was stimulated with pair 2 pulses(interpulse interval was 50 ms) and the EPSC2/ EPSC1 ratio was analyzed. Moreover ,the hold 2 ing potential was changed from - 80 to + 60mV and the current 2 voltage curve was drawn. Results Intralipid didn ’ t affect EPSC ,but 500 μ mol/ L propofol reduced EPSC to (67 1 5 ± 1 1 7) % of the base value after application of propofol. 500 μ mol/ L propofol decreased the EPSC2/ EPSC1 ratio and shif 2 ted the current 2 voltage curve to the left. Conclusion 　 500 μ mol/ L propofol inhibits excitatory synaptic transmission in hippocampal CA1 pyramidal neurons of rats. The activity of GABA A receptor in the presynaptic and postsynaptic membrane may be increased with propofol applied.
关键词	兴奋性突触后电流 Γ2氨基丁酸受体丙泊酚海马
资助者	本课题为国家自然科学基金资助项目(39930080) ; 本课题为国家自然科学基金资助项目(39930080)
收录类别	其他
语种	中文
资助者	本课题为国家自然科学基金资助项目(39930080) ; 本课题为国家自然科学基金资助项目(39930080)
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/1141
专题	科研部门_学习记忆的分子神经机制（徐林）
作者单位	1.广西医科大学附属第一医院麻醉科,南宁530021 2.中国科学院昆明动物研究所学习与记忆实验室,昆明650223 3.华中科技大学同济医学院附属协和医院麻醉科
推荐引用方式 GB/T 7714	谢玉波,刘敬臣,徐林*,等. 丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响[J]. 临床麻醉学杂志,2005,21(6):399-401.
APA	谢玉波,刘敬臣,徐林*,&曾邦雄.(2005).丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响.临床麻醉学杂志,21(6),399-401.
MLA	谢玉波,et al."丙泊酚对大鼠海马CA1 区兴奋性突触传递的影响".临床麻醉学杂志 21.6(2005):399-401.

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