Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C2type Lectin2like Protein Subunits from Daboia russellii siamensis

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	Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C2type Lectin2like Protein Subunits from Daboia russellii siamensis
其他题名	圆斑蝰蛇毒七个C-型凝集素样蛋白亚基的cDNA克隆与序列分析
	Zhong SR 1,2; Jin Y 1; Li DS 1; Wang WY 1; Xiong YL*1; xiongyl@mail.kiz.ac.cn
	2005
发表期刊	动物学研究
卷号	26 期号:4 页码:337-343
合作性质	其它
摘要	Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturer＇s protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector. Fourteen positive clones were selected for nucleotide sequencing and seven cDNAs encoding various snake venom C-type lectin-like protein precursors, designated as DRS-L1, DRS-L2, DRS-L3, DRS-L4 DRS-L5, DRS-L6 and DRS-L7, were obtained. Amino acid sequences of these proteins were deduced and each contains a carbohydrate recognition domain. Of all the deduced protein sequences, only DRS-L1 seemed to represent a closer sequence similarity to α subunits of other known snake venom C-type lectin-like proteins using the BLAST program. Homology comparison combined with analysis of cysteine position indicate that DRS-L1 and DRS-L2 are probably the light chain LC2 and LC1 of factor X activator from Daboia russellii siamensis venom, respectively. DRS-L3 and DRS-L4 might be the β subunits of higher molecular weight C-type lectin-like proteins while DRS-L5 and DRS-L6 might be β subunits of lower molecular weight C-type lectin-like proteins. DRS-L7 might be the β subunit of a platelet membrane glycoprotein Ib-binding protein similar to echicetin.
其他摘要	按照Promega公司的mRNA提取试剂盒操作手册,从圆斑蝰蛇(Daboiarusselliisiamensis)的毒腺中提取mRNA;利用RTPCR的方法进行体外扩增,获得C-型凝集素蛋白的基因,克隆到pMD18T载体中。随机挑选14个阳性克隆进行核酸测序,获得7个编码不同蛇毒C-型凝集素样蛋白亚基的cDNA,分别命名为DRSL1、DRSL2、DRSL3、DRSL4、DRSL5、DRSL6和DRSL7。由基因序列推导出的氨基酸序列表明,克隆到的7个蛇毒C-型凝集素样蛋白的亚基中均有糖识别结构域存在。BLAST分析显示,仅有DRSL1的蛋白序列与目前已知的蛇毒C-型凝集素样蛋白的α亚基相似。序列同源性比较并结合半胱氨酸位点分析,推测DRSL1和DRSL2可能分别是圆斑蝰蛇毒Ⅹ因子激活剂的轻链LC2和LC1。DRSL3和DRSL4可能是高分子量的蛇毒C-型凝集素样蛋白的β亚基,而DRSL5和DRSL6可能是低分子量的蛇毒C-型凝集素样蛋白的β亚基。DRSL7可能是类似于血小板膜糖蛋白Ib结合蛋白的β亚基。
关键词	Daboia Russellii Siamensis Sv 2 Cttls Sv 2 Ctlps Cdna Sequence Analysis
收录类别	其他
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/1192
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	xiongyl@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Zhong SR,Jin Y,Li DS,et al. Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C2type Lectin2like Protein Subunits from Daboia russellii siamensis[J]. 动物学研究,2005,26(4):337-343.
APA	Zhong SR,Jin Y,Li DS,Wang WY,Xiong YL*,&xiongyl@mail.kiz.ac.cn.(2005).Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C2type Lectin2like Protein Subunits from Daboia russellii siamensis.动物学研究,26(4),337-343.
MLA	Zhong SR,et al."Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C2type Lectin2like Protein Subunits from Daboia russellii siamensis".动物学研究 26.4(2005):337-343.

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