哺乳动物胚胎发育（郑萍）知识库

多能干细胞(pluripotent stem cells, PSCs)能无限增殖且具有发育多潜能性, 在再生医

学中有巨大的应用前景, 但长期扩增过程中产生的遗传物质不稳定性及潜在致瘤性阻碍了临床应

用。深入理解多能干细胞维持遗传物质稳定性的方式和机制具有重要的理论价值和应用前景。由

于功能的特殊性, 相较于体细胞, 多能干细胞维持遗传物质稳定性的能力更强, 并有其独特的机制。该文简要综述了近年来在多能干细胞维持遗传物质稳定性方面的研究进展, 包括干细胞如何有效应对DNA复制压力、如何有效修复DNA损伤以及在DNA损伤情况下的细胞命运决定机制。

Pluripotent stem cells (PSCs) hold great promise for cell-based regenerative medicine. However, genomic instability and tumorigenicity hamper their full applications. Understanding the mechanisms that regulate their genome stability is critical to address this issue. Due to the specific functional purpose, PSCs possess high competence to safeguard genomic stability by employing specific strategies. In this review, we summarized the current progress in understanding the mechanisms that the PSCs utilize to cope with DNA replication stress, to repair DNA damage, and to determine the cell fates after unrepairable damage.