Isoharringtonine inhibits breast cancer stem-like properties and STAT3 signaling.

doi:10.1016/j.biopha.2018.04.076

	Isoharringtonine inhibits breast cancer stem-like properties and STAT3 signaling.
	Wei Chen 1,2; Baowei Jiao 2; Mei Cheng 2,3; Ling Ni 4; Li Zou 2; Qin Yang 2; Xianghai Cai 4
	2018
发表期刊	Biomedicine & Pharmacotherapy
期号	103 页码:435–442
摘要	Objectives: Breast cancer stem cells (BCSCs) contribute to breast cancer progression, relapse, and treatmentresistance. Identification of the natural inhibitory components of BCSCs is therefore critical for clinical treat-ment. Here, we investigated whether isoharringtonine (IHT) had inhibitory effects on BCSCs in breast cancer celllines. Methods: HCC1806, HCC1937, and MCF7 cells were treated with IHT. The proliferation and the migration ofcells were detected by MTS assay and wound healing migration assay, respectively. The proportions of BCSCswere determined by flow cytometry and tumor sphere formation assay. Using real-time quantitative polymerasechain reaction (qRT-PCR) and Western blotting, the expression of Nanog and activation of STAT3 were detected,respectively. Results: Results showed that IHT inhibited the proliferation of HCC1806, HCC1937, and MCF-7 cells, andsuppressed the migration of HCC1806 and HCC1937 cells in a dose-dependent manner. IHT treatment decreasedthe proportion of BCSCs in MCF-7, HCC1806, and HCC1937 cells. In addition, the mRNA level of Nanong wassignificantly downregulated after IHT treatment. We also found an inhibitory effect of IHT on STAT3 activation. Conclusion: IHT inhibited the proliferation, migration, and BCSC proportion of breast cancer cell lines via in-hibition of the STAT3/Nanong pathway.
关键词	Triple-negative Breast Cancer Cancer Stem Cells Isoharringtonine Stat3
DOI	10.1016/j.biopha.2018.04.076
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12228
专题	遗传资源与进化国家重点实验室
通讯作者	Xianghai Cai
作者单位	1.Institute of Physical Science and Information Technology, Anhui University, 230601, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China 3.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, 650223, China 4.Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China
推荐引用方式 GB/T 7714	Wei Chen,Baowei Jiao,Mei Cheng,et al. Isoharringtonine inhibits breast cancer stem-like properties and STAT3 signaling.[J]. Biomedicine & Pharmacotherapy,2018(103):435–442.
APA	Wei Chen.,Baowei Jiao.,Mei Cheng.,Ling Ni.,Li Zou.,...&Xianghai Cai.(2018).Isoharringtonine inhibits breast cancer stem-like properties and STAT3 signaling..Biomedicine & Pharmacotherapy(103),435–442.
MLA	Wei Chen,et al."Isoharringtonine inhibits breast cancer stem-like properties and STAT3 signaling.".Biomedicine & Pharmacotherapy .103(2018):435–442.

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