Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis

	Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis
	Cheng, RM; Tang, XP; Long, AL; Mwangi, J; Lai, R; Sun, RP; Long, CB; Zhang, ZQ
	2019
发表期刊	ZOOLOGICAL RESEARCH
ISSN	2095-8137
卷号	40 期号:3 页码:205-210
摘要	Protease inhibitors have been reported rarely from the leech Hirudinaria manillensis. In this study, we purified a novel protease inhibitor (bdellin-HM-2) with anticoagulant properties from H. manillensis. With a molecular weight of 1.4x104, bdellin-HM-2 was also characterized with three intra-molecular disulfide bridges at the N-terminus and multiple HHXDD and HXDDmotifs at the C-terminus. cDNAcloning revealed that the putative nucleotide-encoding protein of bdellin-HM-2 contained 132 amino acids and was encoded by a 399 bp open reading frame (ORF). Sequence alignment showed that bdellin-HM-2 shared similarity with the"non-classical" Kazal-type serine protease inhibitors, but had no inhibitory effect on trypsin, elastase, chymotrypsin, kallikrein, factor XIIa (FXIIa), factor XIa (FXIa), factor Xa (FXa), thrombin, or plasmin. Bdellin-HM-2 showed anticoagulant effects by prolonging the activated partial thromboplastin time (aPTT), indicating a role in enabling H. manillensis to obtain a blood meal from its host. Our results suggest that bdellin-HM-2 may play a crucial role in blood-sucking in this leech species and may be a potential candidate for the development of clinical anti-thrombotic drugs.
收录类别	SCIE
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12445
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
推荐引用方式 GB/T 7714	Cheng, RM,Tang, XP,Long, AL,et al. Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis[J]. ZOOLOGICAL RESEARCH,2019,40(3):205-210.
APA	Cheng, RM.,Tang, XP.,Long, AL.,Mwangi, J.,Lai, R.,...&Zhang, ZQ.(2019).Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis.ZOOLOGICAL RESEARCH,40(3),205-210.
MLA	Cheng, RM,et al."Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis".ZOOLOGICAL RESEARCH 40.3(2019):205-210.

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