High concentration of sugars is genotoxic to folate-deficient cells

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	High concentration of sugars is genotoxic to folate-deficient cells
	Guo, XH; Dai, XQ; Ni, J; Cao, N; Yang, GF; Xue, JL; Wang, X
	2019
发表期刊	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
ISSN	0027-5107
卷号	814 页码:15-22
摘要	Patients with type 2 diabetes mellitus (T2DM) are associated with an elevated, but poorly understood baseline of genomic instability (GIN). Expert panels are still debating on whether hyperglycemia is the key element in conferring this high GIN. Since high blood glucose and low blood folate are prevalent in T2DM, we hypothesized that high glucose may work with low folate to induce GIN. Using NCM460, CCD841 and L02 cell lines as in vitro cell models, we investigated the genotoxic effects of high sugars (HS; 1-2% glucose, fructose, galactose or sucrose) alone or in combination with folate deficiency (23 nM, FD) over a course of 7 days by the cytokinesis block micronucleus assay. We found that HS is nongenotoxic to NCM460, CCD841 and L02 cells. However, the combination of HS and FD induced significantly higher levels of micronuclei, nucleoplasmic bridges and nuclear buds. Our in vitro work demonstrates that HS is non-genotoxic under folate repletive condition, but is genotoxic under FD condition. These results provide preclinal proof of concept that concomitant hyperglycemia and low folate may explain, at least in part, the high baseline of GIN in T2DM patients, suggesting that folate levels should be kept under control in order to limit the risk of GIN and carcinogenesis in T2DM.
收录类别	SCIE
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12529
专题	科研部门_肿瘤生物学（陈策实）
推荐引用方式 GB/T 7714	Guo, XH,Dai, XQ,Ni, J,et al. High concentration of sugars is genotoxic to folate-deficient cells[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2019,814:15-22.
APA	Guo, XH.,Dai, XQ.,Ni, J.,Cao, N.,Yang, GF.,...&Wang, X.(2019).High concentration of sugars is genotoxic to folate-deficient cells.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,814,15-22.
MLA	Guo, XH,et al."High concentration of sugars is genotoxic to folate-deficient cells".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 814(2019):15-22.

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