中国科学院昆明动物研究所机构知识库

由细菌分泌的β孔道形成毒素类型的产气单胞菌溶素在细菌感染方面行使重要作用。但是，在包括多种植物和动物在内的非有毒物种中，存在着大量的产气单胞菌溶素类似蛋白。我们之前的研究表明,含有产气单胞菌溶素结构域的蛋白质复合体βγ-CAT能够帮助宿主对抗细菌感染。在线虫模式生物中，lin-24这种基因编码了只含有一个产气单胞菌溶素结构域，而不与其他结构域融合。但是它的生理功能仍然未知。通过构建GFP转基因线虫，我们发现lin-24主要表达在消化道，表明lin-24在抗微生物感染反应中可能起到作用。我们探索了线虫的表型，结果表明，与对照组相比，干扰组线虫的寿命和产卵量均显著降低。与此同时，干扰组更容易被绿脓杆菌感染。因此lin-24可能在宿主抗微生物免疫中发挥重要作用。由于lin-24被敲除后，线虫看起来似乎更苗条，于是其脂滴和脂质含量的变化被观测。结果表明，与对照组相比，脂滴的大小降低。转录组结果显示，与对照组相比，lin-24敲除的干扰组虫子表现出显著的基因表达差异。KEGG映射聚集了许多通路途径，揭示了差异基因显著富集于“溶酶体通路” 。我们目前的研究揭示了LIN-24的关键作用，在线虫中扮演重要的生理角色。它与溶酶体功能、脂质代谢和抗微生物感染有关。

Aerolysins are bacterial β-pore-forming toxins responsible for large proportion of bacterial infection. But numerous aerolysin-like proteins (ALPs) exist in non-toxic species including many type of plants and animals. Our previous study showed that aerolysin-domain containing protein complex βγ-CAT help the host fight against bacterial infection. In the model organism Caenorhabditis elegans, A gene lin-24 encodes a protein containing only one aerolysin domain without fusion with other domains. But its physiological function remains unknown. By generating GFP reporter transgenic worm, we found that lin-24 mainly expressed the digestive tract, indicating a possible role in anti-microbial infection response. we explored the phenotype of Caenorhabditis elegans .. The results showed that the life span and egg laying amount of the knock-down group of Caenorhabditis elegans were significantly reduced compared with those of control group. Meanwhile, the knock-down group was more susceptible to bacterium Pseudomonas aeruginosa infection. Thus, lin-24 may play an important role in host anti-microbial immunity. Since the lin-24 knocked down worms seems more slimmer, the change of lipid droplet and lipid content were assessed.The result showed that the size of lipid droplets was decreased compared with that of the control group.RNA-seq of lin-24 knock-down worms showed remarkable variation of gene expression pattern as compared with that of control worms. KEGG mapping clustered many pathways, revealing that “lysosome pathway” was the most significantly enriched. Our present study revealed pivotal role of LIN-24. It plays pivotal physiological roles in the worm. And it linked the lysosomal function, lipid metabolism and anti-microbial infection.