miR145 Regulates the Proliferation and Apoptosis of Rat Vascular Endothelial Cells under Hyperglycemia by Targeting the ANGPT2 Gene and Involving the NF kappa B Signaling Pathway

	miR145 Regulates the Proliferation and Apoptosis of Rat Vascular Endothelial Cells under Hyperglycemia by Targeting the ANGPT2 Gene and Involving the NF kappa B Signaling Pathway
	Zhang, Wen; Tang, Xin-Hua; Zhang, Jin-Juan; He, Quan
	2020
发表期刊	DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY
ISSN	1178-7007
卷号	13 页码:4435-4446
摘要	Purpose: A majority of diabetes mellitus patients with disturbances of glucose metabolism present with vascular complications. This study aimed to explore regulatory mechanisms of miR145 and its potential target gene ANGPT2 on diabetic vasculopathy under hyperglycemia.Methods: Based on the fact that miR145 is detected in rat aortic endothelial cells (RAECs) under hyperglycemia, RAECs were transfected with miR145 mimics/inhibitor for further confirmation. RAEC proliferation was detected with CCK8 assays, and cell apoptosis and CD34(+)-cell population with annexinV-PI staining and anti-CD34FITC on flow cytometry, respectively. Then, qPCR and Western blot were applied to detect mRNA and protein expression of ANGPT2 and involved pathway factor NF kappa B p65. Subsequently, dual lucifer-ase-reporter gene analysis was utilized to verify whether miR145 acted directly upon the 3'UTR of ANGPT2 mRNA.Results: The ANGPT2 gene was confirmed to be a direct target of miR145. miR145 mimics markedly downregulated the expression of ANGPT2 and NF kappa B p65, boosted the percentage of the CD34+ phenotype, and promoted proliferation and suppressed apoptosis of RAECs under hyperglycemia.Conclusion: miR145 might regulate the viability of RAECs via targeting ANGPT2 and involving NF kappa B signaling to exert a protective effect on diabetic vasculature.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12675
专题	科研部门_哺乳动物胚胎发育（郑萍）
推荐引用方式 GB/T 7714	Zhang, Wen,Tang, Xin-Hua,Zhang, Jin-Juan,et al. miR145 Regulates the Proliferation and Apoptosis of Rat Vascular Endothelial Cells under Hyperglycemia by Targeting the ANGPT2 Gene and Involving the NF kappa B Signaling Pathway[J]. DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY,2020,13:4435-4446.
APA	Zhang, Wen,Tang, Xin-Hua,Zhang, Jin-Juan,&He, Quan.(2020).miR145 Regulates the Proliferation and Apoptosis of Rat Vascular Endothelial Cells under Hyperglycemia by Targeting the ANGPT2 Gene and Involving the NF kappa B Signaling Pathway.DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY,13,4435-4446.
MLA	Zhang, Wen,et al."miR145 Regulates the Proliferation and Apoptosis of Rat Vascular Endothelial Cells under Hyperglycemia by Targeting the ANGPT2 Gene and Involving the NF kappa B Signaling Pathway".DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY 13(2020):4435-4446.

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