HSV-1 infection and pathogenesis in the tree shrew eye following corneal inoculation

	HSV-1 infection and pathogenesis in the tree shrew eye following corneal inoculation
	Li, Lihong; Li, Yan; Li, Xin; Xia, Yujie; Wang, Erlin; Gong, Daohua; Chen, Guijun; Yang, Liping; Zhang, Ke; Zhao, Zhuanghong; Fraser, Nigel W.; Fan, Quanshui; Li, Bing; Zhang, Hui; Cao, Xia; Zhou, Jumin
	2020
发表期刊	JOURNAL OF NEUROVIROLOGY
ISSN	1355-0284
卷号	26 期号:3 页码:391-403
摘要	Herpes simplex virus type I (HSV-1) infection causes inflammation in the cornea known as herpes simplex virus keratitis (HSK), a common but serious corneal disease. It is not entirely clear whether the virus during recurring infection comes from the trigeminal ganglia or the eye tissue, including the retina and ciliary ganglion. Because the tree shrew is closely related to primates and tree shrew eye anatomic structures are similar to humans, we studied HSV-1 corneal infection in the tree shrew. We found that HSK symptoms closely mimic those found in human HSK showing typical punctiform and dendritic viral keratitis during the acute infection period. Following the HSV-specific lesions, complications such as stromal scarring, corneal thickening (primary infection), opacity, and neovascularization were observed. In the tree shrew model, following ocular inoculation, the cornea becomes infected, and viral protein can be detected using anti-HSV-1 antibodies in the epithelial layer and retina neuronal ganglion cells. The HSV-1 transcripts, ICP0, ICP4, and LAT can be detected at 3 days post-infection (dpi), peaking at 5 dpi. After 2 weeks, ICP4 and ICP0 transcripts are reduced to a basal level, but the Latency Associated Transcripts (LATs) continue to accumulate. Interestingly, after the acute infection, we still detected abundant active HSV-1 in tree shrew eyes. Further, we found HSV-1 persistent in the ciliary ganglion and cornea. These findings are discussed in support of the tree shrew as a non-human primate HSK model, which could be useful for mechanistic studies of HSK.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12676
专题	科研部门_基因调控与表达遗传（周巨民）
推荐引用方式 GB/T 7714	Li, Lihong,Li, Yan,Li, Xin,et al. HSV-1 infection and pathogenesis in the tree shrew eye following corneal inoculation[J]. JOURNAL OF NEUROVIROLOGY,2020,26(3):391-403.
APA	Li, Lihong.,Li, Yan.,Li, Xin.,Xia, Yujie.,Wang, Erlin.,...&Zhou, Jumin.(2020).HSV-1 infection and pathogenesis in the tree shrew eye following corneal inoculation.JOURNAL OF NEUROVIROLOGY,26(3),391-403.
MLA	Li, Lihong,et al."HSV-1 infection and pathogenesis in the tree shrew eye following corneal inoculation".JOURNAL OF NEUROVIROLOGY 26.3(2020):391-403.

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