Longitudinal transcriptomic characterization of viral genes in HSV-1 infected tree shrew trigeminal ganglia

	Longitudinal transcriptomic characterization of viral genes in HSV-1 infected tree shrew trigeminal ganglia
	Wang, Erlin; Ye, Yunshuang; Zhang, Ke; Yang, Jinlong; Gong, Daohua; Zhang, Jianhua; Hong, Renjun; Zhang, Huan; Li, Lihong; Chen, Guijun; Yang, Liping; Liu, Jianmei; Cao, Hanyu; Du, Ting; Fraser, Nigel W.; Cheng, Le; Cao, Xia; Zhou, Jumin
	2020
发表期刊	VIROLOGY JOURNAL
卷号	17 期号:1
摘要	Background Following acute infection, Herpes Simplex virus-1 (HSV-1) establishes lifelong latency and recurrent reactivation in the sensory neurons of trigeminal ganglia (TG). Infected tree shrew differs from mouse and show characteristics similar to human infection. A detailed transcriptomic analysis of the tree shrew model could provide mechanistic insights into HSV-1 infection in humans. Methods We sequenced the transcriptome of infected TGs from tree shrews and mice, and 4 human donors, then examined viral genes expression up to 58 days in infected TGs from mouse and tree shrew, and compare the latency data with that in human TGs. Results Here, we found that all HSV-1 genes could be detected in mouse TGs during acute infection, but 22 viral genes necessary for viral transcription, replication and viral maturation were not expressed in tree shrew TGs during this stage. Importantly, during latency, we found that LAT could be detected both in mouse and tree shrew, but the latter also has an ICP0 transcript signal absent in mouse but present in human samples. Importantly, we observed that infected human and tree shrew TGs have a more similar LAT region transcription peak. More importantly, we observed that HSV-1 spontaneously reactivates from latently infected tree shrews with relatively high efficiency. Conclusions These results represent the first longitudinal transcriptomic characterization of HSV-1 infection in during acute, latency and recurrent phases, and revealed that tree shrew infection has important similar features with human infection.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12679
专题	科研部门_基因调控与表达遗传（周巨民）
推荐引用方式 GB/T 7714	Wang, Erlin,Ye, Yunshuang,Zhang, Ke,et al. Longitudinal transcriptomic characterization of viral genes in HSV-1 infected tree shrew trigeminal ganglia[J]. VIROLOGY JOURNAL,2020,17(1).
APA	Wang, Erlin.,Ye, Yunshuang.,Zhang, Ke.,Yang, Jinlong.,Gong, Daohua.,...&Zhou, Jumin.(2020).Longitudinal transcriptomic characterization of viral genes in HSV-1 infected tree shrew trigeminal ganglia.VIROLOGY JOURNAL,17(1).
MLA	Wang, Erlin,et al."Longitudinal transcriptomic characterization of viral genes in HSV-1 infected tree shrew trigeminal ganglia".VIROLOGY JOURNAL 17.1(2020).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2021102504.pdf（2333KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文