A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection

	A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection
	Wang, Peng; Luo, Ronghua; Zhang, Min; Wang, Yaqing; Song, Tianzhang; Tao, Tingting; Li, Zhongyu; Jin, Lin; Zheng, Hongyi; Chen, Wenwen; Zhao, Mengqian; Zheng, Yongtang; Qin, Jianhua
	2020
发表期刊	CELL DEATH & DISEASE
ISSN	2041-4889
卷号	11 期号:12
摘要	COVID-19, caused by SARS-CoV-2, is an acute and rapidly developing pandemic, which leads to a global health crisis. SARS-CoV-2 primarily attacks human alveoli and causes severe lung infection and damage. To better understand the molecular basis of this disease, we sought to characterize the responses of alveolar epithelium and its adjacent microvascular endothelium to viral infection under a co-culture system. SARS-CoV-2 infection caused massive virus replication and dramatic organelles remodeling in alveolar epithelial cells, alone. While, viral infection affected endothelial cells in an indirect manner, which was mediated by infected alveolar epithelium. Proteomics analysis and TEM examinations showed viral infection caused global proteomic modulations and marked ultrastructural changes in both epithelial cells and endothelial cells under the co-culture system. In particular, viral infection elicited global protein changes and structural reorganizations across many sub-cellular compartments in epithelial cells. Among the affected organelles, mitochondrion seems to be a primary target organelle. Besides, according to EM and proteomic results, we identified Daurisoline, a potent autophagy inhibitor, could inhibit virus replication effectively in host cells. Collectively, our study revealed an unrecognized cross-talk between epithelium and endothelium, which contributed to alveolar-capillary injury during SARS-CoV-2 infection. These new findings will expand our understanding of COVID-19 and may also be helpful for targeted drug development.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12682
专题	科研部门_分子免疫药理学（郑永唐）
推荐引用方式 GB/T 7714	Wang, Peng,Luo, Ronghua,Zhang, Min,et al. A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection[J]. CELL DEATH & DISEASE,2020,11(12).
APA	Wang, Peng.,Luo, Ronghua.,Zhang, Min.,Wang, Yaqing.,Song, Tianzhang.,...&Qin, Jianhua.(2020).A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.CELL DEATH & DISEASE,11(12).
MLA	Wang, Peng,et al."A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection".CELL DEATH & DISEASE 11.12(2020).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2021102503.pdf（6841KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文