A secreted pore-forming protein modulates cellular endolysosomes to augment antigen presentation

	A secreted pore-forming protein modulates cellular endolysosomes to augment antigen presentation
	Deng, Cheng-Jie; Liu, Long; Liu, Ling-Zhen; Wang, Qi-Quan; Guo, Xiao-Long; Lee, Wen-Hui; Li, Sheng-An; Zhang, Yun
	2020
发表期刊	FASEB JOURNAL
ISSN	0892-6638
卷号	34 期号:10 页码:13609-13625
摘要	Bacterial pore-forming toxin aerolysin-like proteins are widely distributed in animals and plants. Emerging evidence supports their roles in host innate immunity, but their direct actions in adaptive immunity remain elusive. In this study, we found that beta gamma-CAT, an aerolysin-like protein and trefoil factor complex identified in the frogBombina maxima, modulated several steps of endocytic pathways during dendritic cell antigen presentation. The protein augmented the antigen uptake of dendritic cells and actively neutralized the acidification of cellular endocytic organelles to favor antigen presentation. In addition, the release of functional exosome-like extracellular vesicles was largely enhanced in the presence of beta gamma-CAT. The cellular action of beta gamma-CAT increased the number of major histocompatibility complex (MHC) I-ovalbumin and MHC II molecules on dendritic cell surfaces and the released exosome-like extracellular vesicles. An enhanced antigen presentation capacity of dendritic cell for priming of naive T cells was detected in the presence of beta gamma-CAT. Collectively, these effects led to strong cytotoxic T lymphocyte responses and antigen-specific antibody responses. Our findings provide evidence that a vertebrate-secreted pore-forming protein can augment antigen presentation by directly modulating cellular endocytic and exocytic pathways, leading to robust activation of adaptive immunity.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12696
专题	科研部门_生物毒素与人类疾病（张云）
推荐引用方式 GB/T 7714	Deng, Cheng-Jie,Liu, Long,Liu, Ling-Zhen,et al. A secreted pore-forming protein modulates cellular endolysosomes to augment antigen presentation[J]. FASEB JOURNAL,2020,34(10):13609-13625.
APA	Deng, Cheng-Jie.,Liu, Long.,Liu, Ling-Zhen.,Wang, Qi-Quan.,Guo, Xiao-Long.,...&Zhang, Yun.(2020).A secreted pore-forming protein modulates cellular endolysosomes to augment antigen presentation.FASEB JOURNAL,34(10),13609-13625.
MLA	Deng, Cheng-Jie,et al."A secreted pore-forming protein modulates cellular endolysosomes to augment antigen presentation".FASEB JOURNAL 34.10(2020):13609-13625.

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