Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms

	Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms
	Lin, Bo; Zhang, Jia-Rui; Lu, Hui-Juan; Zhao, Lin; Chen, Jing; Zhang, Hong-Fei; Wei, Xue-Song; Zhang, Liang-Yu; Wu, Xiao-Bing; Lee, Wen-Hui
	2020
发表期刊	PLOS NEGLECTED TROPICAL DISEASES
ISSN	1935-2735
卷号	14 期号:11
摘要	Bungarus multicinctus is the most venomous snake distributed in China and neighboring countries of Myanmar, Laos, north Vietnam and Thailand. The high mortality rate of B. multicinctus envenomation is attributed to the lethal components of alpha-, beta-, gamma- and kappa- bungarotoxins contained in the venom. Although anti-B. multicinctus sera were produced in Shanghai, Taiwan and Vietnam, the most widely clinic used product was term as B. multicinctus antivenin and manufactured by Shanghai Serum Bio-technology Co. Ltd. In the present investigation, high purity alpha-, beta- and gamma-bungarotoxins were separately isolated from B. multicinctus crude venom. Rabbit anti- alpha-, beta- and gamma-bungarotoxin antisera were prepared by common methods, respectively. LD50 values of alpha-, beta- and gamma-bungarotoxins were systematically determined via three administration pathways (intraperitoneal, intramuscular and intravenous injections) in Kunming mice. LD50 values of beta-bungarotoxin were closely related with injection routines but those of both alpha- and gamma-bungarotoxins were not dependent on the injection routines. Commercial B. multicinctus antivenin showed strong immunoreaction with high molecular weight fractions of the B. multicinctus but weakly recognized low molecular weight fractions like alpha- and gamma-bungarotoxins. Although B. multicinctus antivenin showed immunoreaction with high molecular weight fractions of Bungarus fasciatus, Naja atra, Ophiophagus hannah venoms but the antivenin only demonstrated animal protection efficacy against O. hannah venom. These results indicated that the high molecular weight fractions of the O. hannah played an important role in venom lethality but those of B. fasciatus and N. atra did not have such a role.Author summarySnakebite envenoming is an important public health problem around the world. Bungarus multicinctus envenomation is regarded as most dangerous animal bitten disease in China and neighboring countries. At present, the most widely clinic used B. multicinctus antisera was term as B. multicinctus antivenin and manufactured by Shanghai Serum Bio-technology Co. Ltd. By using high purity alpha-, beta- and gamma-bungarotoxins isolated from B. multicinctus crude venom and prepared corresponding rabbit anti- alpha-, beta- and gamma-bungarotoxin antisera, we evaluated the efficacy of B. multicinctus antivenin against the crude venom, isolated bungarotoxins, as well as other 8 medical important Chinese venomous snake venoms. B. multicinctus antivenin showed strong immunoreaction with high molecular weight fractions of the B. multicinctus venom but weakly recognized low molecular weight fractions like alpha- and gamma-bungarotoxins. Moreover, B. multicinctus antivenin can neutralize Ophiophagus hannah venom but not B. fasciatus venom was demonstrated.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12698
专题	科研部门_生物毒素与人类疾病（张云）
推荐引用方式 GB/T 7714	Lin, Bo,Zhang, Jia-Rui,Lu, Hui-Juan,et al. Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms[J]. PLOS NEGLECTED TROPICAL DISEASES,2020,14(11).
APA	Lin, Bo.,Zhang, Jia-Rui.,Lu, Hui-Juan.,Zhao, Lin.,Chen, Jing.,...&Lee, Wen-Hui.(2020).Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms.PLOS NEGLECTED TROPICAL DISEASES,14(11).
MLA	Lin, Bo,et al."Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms".PLOS NEGLECTED TROPICAL DISEASES 14.11(2020).

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