Osteoking downregulates Mgp in an osteoporotic fracture rat model

	Osteoking downregulates Mgp in an osteoporotic fracture rat model
	Sun Yan; Wang Xiaoqi; Chen Ran; Zhu Di; Shen Zhiqiang; Zhao Hongbin; Lee Wenhui
	2020
发表期刊	JOURNAL OF TRADITIONAL CHINESE MEDICINE
ISSN	0255-2922
卷号	40 期号:3 页码:422-431
摘要	OBJECTIVE: To investigate the effectiveness of osteoking, a Traditional Chinese Medicine originating from Yi nationality, against osteoporosis (OP) and osteoporotic fracture (OPF), and to elucidate its mechanism of action.METHODS: An osteoporotic fracture rat model was established; animals were divided into three treatment groups: parathyroid hormone, osteoking and 0.9%NaCI. After 4, 8 and 12 weeks of treatment, serum and bone tissues were collected. Enzyme-linked immuno sorbent assay, x-ray, histopathological evaluation and proteomics were used. Proteomics and GO annotation were performed based on identified peptides. The relative network was obtained from the STRING database and verified by polymerase chain reaction and Western blotting.RESULTS: After osteoking treatment, the bone mineral density (BMD) increased with time in the osteoking group. At week 12, the BMD and bone mineral salt content of the osteoking group were 4.5% and 20.6% higher than those of the negative control group, respectively. Furthermore, the body weight followed the order of positive control group > osteoking group > negative control group, with significant differences among the groups (P < 0.05). Micro-CT analysis of femur sections revealed that the bone surface/volume ratio was significantly higher in the osteoking group than that in the negative control group. X-ray images demonstrated that the osteoking group showed clear callus. Moreover, high-voltage micro-CT demonstrated a massive cortical bone accumulation in the osteoking group. The gray values of callus in the osteoking group were higher than those in the negative group. From week 4 to 12, the serum bone alkaline phosphatase level increased by 49.6% in the osteoking group and the serum propeptide of type I procollagen level decreased by 80.6%. Alizarin red staining demonstrated that the calcium deposition in the osteoking group was higher than that in the negative control group. Notably, the expression of Mgp, a key osteogenesis inhibitor, was lower in the osteoking group compared with the negative control group. Moreover, Sparc, bone morphogenetic protein-2 and Bglap expression was higher in the osteoking group through activation of the transforming growth factor-receptor activator of nuclear factor kappa B Ligand pathway.CONCLUSION: Osteoking treatment increased bone quality and promoted calcium deposition. The results suggest that osteoking inhibits Mgp through the TGF-beta/RANKL pathway to improve OP/OPF. (C) 2020 JTCM. All rights reserved.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12702
专题	科研部门_生物毒素与人类疾病（张云）
推荐引用方式 GB/T 7714	Sun Yan,Wang Xiaoqi,Chen Ran,et al. Osteoking downregulates Mgp in an osteoporotic fracture rat model[J]. JOURNAL OF TRADITIONAL CHINESE MEDICINE,2020,40(3):422-431.
APA	Sun Yan.,Wang Xiaoqi.,Chen Ran.,Zhu Di.,Shen Zhiqiang.,...&Lee Wenhui.(2020).Osteoking downregulates Mgp in an osteoporotic fracture rat model.JOURNAL OF TRADITIONAL CHINESE MEDICINE,40(3),422-431.
MLA	Sun Yan,et al."Osteoking downregulates Mgp in an osteoporotic fracture rat model".JOURNAL OF TRADITIONAL CHINESE MEDICINE 40.3(2020):422-431.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2021110107.pdf（3701KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文