Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus

	Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus
	Fotakis, Anna K.; Denham, Sean D.; Mackie, Meaghan; Orbegozo, Miren Iraeta; Mylopotamitaki, Dorothea; Gopalakrishnan, Shyam; Sicheritz-Ponten, Thomas; Olsen, Jesper V.; Cappellini, Enrico; Zhang, Guojie; Christophersen, Axel; Gilbert, M. Thomas P.; Vagene, Ashild J.
	2020
发表期刊	PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
ISSN	0962-8436
卷号	375 期号:1812
摘要	Mineralized dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. Here we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of sixteenth-century human dental calculus from an individual from Trondheim, Norway. When phylogenetically placed, this genome falls in branch 3I among the diversity of other contemporary ancient strains from Northern Europe. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. Mycobacterium leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual's dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early-stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record.This article is part of the theme issue 'Insights into health and disease from ancient biomolecules'.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12725
专题	科研部门_遗传多样性基因组学研究组（张国捷）
推荐引用方式 GB/T 7714	Fotakis, Anna K.,Denham, Sean D.,Mackie, Meaghan,et al. Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,2020,375(1812).
APA	Fotakis, Anna K..,Denham, Sean D..,Mackie, Meaghan.,Orbegozo, Miren Iraeta.,Mylopotamitaki, Dorothea.,...&Vagene, Ashild J..(2020).Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus.PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,375(1812).
MLA	Fotakis, Anna K.,et al."Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus".PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 375.1812(2020).

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