A draft genome sequence of the elusive giant squid, Architeuthis dux

	A draft genome sequence of the elusive giant squid, Architeuthis dux
	da Fonseca, Rute R.; Couto, Alvarina; Machado, Andre M.; Brejova, Brona; Albertin, Carolin B.; Silva, Filipe; Gardner, Paul; Baril, Tobias; Hayward, Alex; Campos, Alexandre; Ribeiro, Angela M.; Barrio-Hernandez, Inigo; Hoving, Henk-Jan; Tafur-Jimenez, Ricardo; Chu, Chong; Frazao, Barbara; Petersen, Bent; Penaloza, Fernando; Musacchia, Francesco; Alexander, Graham C., Jr.; Osorio, Hugo; Winkelmann, Inger; Simakov, Oleg; Rasmussen, Simon; Rahman, M. Ziaur; Pisani, Davide; Vinther, Jakob; Jarvis, Erich; Zhang, Guojie; Strugnell, Jan M.; Castro, L. Filipe C.; Fedrigo, Olivier; Patricio, Mateus; Li, Qiye; Rocha, Sara; Antunes, Agostinho; Wu, Yufeng; Ma, Bin; Sanges, Remo; Vinar, Tomas; Blagoev, Blagoy; Sicheritz-Ponten, Thomas; Nielsen, Rasmus; Gilbert, M. Thomas P.
	2020
发表期刊	GIGASCIENCE
ISSN	2047-217X
卷号	9 期号:1
摘要	Background: The giant squid (Architeuthis dux; Steenstrup, 1857) is an enigmatic giant mollusc with a circumglobal distribution in the deep ocean, except in the high Arctic and Antarctic waters. The elusiveness of the species makes it difficult to study. Thus, having a genome assembled for this deep-sea-dwelling species will allow several pending evolutionary questions to be unlocked. Findings: We present a draft genome assembly that includes 200 Gb of Illumina reads, 4 Gb of Moleculo synthetic long reads, and 108 Gb of Chicago libraries, with a final size matching the estimated genome size of 2.7 Gb, and a scaffold N50 of 4.8 Mb. We also present an alternative assembly including 27 Gb raw reads generated using the Pacific Biosciences platform. In addition, we sequenced the proteome of the same individual and RNA from 3 different tissue types from 3 other species of squid (Onychoteuthis banksii, Dosidicus gigas, and Sthenoteuthis oualaniensis) to assist genome annotation. We annotated 33,406 protein-coding genes supported by evidence, and the genome completeness estimated by BUSCO reached 92%. Repetitive regions cover 49.17% of the genome. Conclusions: This annotated draft genome of A. dux provides a critical resource to investigate the unique traits of this species, including its gigantism and key adaptations to deep-sea environments.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12738
专题	科研部门_遗传多样性基因组学研究组（张国捷）
推荐引用方式 GB/T 7714	da Fonseca, Rute R.,Couto, Alvarina,Machado, Andre M.,et al. A draft genome sequence of the elusive giant squid, Architeuthis dux[J]. GIGASCIENCE,2020,9(1).
APA	da Fonseca, Rute R..,Couto, Alvarina.,Machado, Andre M..,Brejova, Brona.,Albertin, Carolin B..,...&Gilbert, M. Thomas P..(2020).A draft genome sequence of the elusive giant squid, Architeuthis dux.GIGASCIENCE,9(1).
MLA	da Fonseca, Rute R.,et al."A draft genome sequence of the elusive giant squid, Architeuthis dux".GIGASCIENCE 9.1(2020).

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2021102516.pdf（943KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文