A positive feedback self-regulatory loop between miR-210 and HIF-1 alpha mediated by CPEB2 is involved in trophoblast syncytialization: implication of trophoblast malfunction in preeclampsia

KIZ OpenIR > 科研部门 > 疾病机理遗传学和进化医学学科组（姚永刚）

	A positive feedback self-regulatory loop between miR-210 and HIF-1 alpha mediated by CPEB2 is involved in trophoblast syncytialization: implication of trophoblast malfunction in preeclampsia
	Wang, Hao; Zhao, Yangyu; Luo, Rongcan; Bian, Xiaotao; Wang, Yongqing; Shao, Xuan; Li, Yu-Xia; Liu, Ming; Wang, Yan-Ling
	2020
发表期刊	BIOLOGY OF REPRODUCTION
ISSN	0006-3363
卷号	102 期号:3 页码:560-570
摘要	The pregnancy complication preeclampsia is directly associated with hypoxic stress and insufficient trophoblast cell differentiation. The hypoxia-inducible microRNA (miRNA), miR-210, has been identified as a significantly up-regulated miRNA in preeclamptic placenta, and evidence in other cell types has indicated a feedback regulation between miR-210 and hypoxia-inducible factor-1 alpha (HIF-1 alpha) under hypoxic condition. It remains unclear whether and how the feedback loop between miR-210 and HIF-1 alpha may contribute to trophoblast dysfunction in preeclampsia. Here, we proved that cytoplasmic polyadenylation element-binding 2 (CPEB2) was a direct target of miR-210 in human trophoblast. CPEB2 could inhibit the translation of hypoxia-induced HIF-1 alpha via directly binding the cytoplasmic polyadenylation element (CPE) site in the 3-untranslated region (UTR) of HIF-1 alpha mRNA. The increase in the HIF-1 alpha level upon hypoxia treatment could be efficiently reversed by miR-210 inhibitor. In addition, CPEB2 was primarily expressed in villous syncytiotrophoblasts, and the suppression of trophoblast cell syncytialization by miR-210 could be significantly rescued by CPEB2 overexpression. In preeclamptic placenta, the expression of CPEB2 was evidently lower than normal pregnant control, and the miR-210 level was aberrantly higher and trophoblast syncytialization was limited. The findings revealed a positive feedback loop between miR-210 and HIF-1 alpha that is mediated by CPEB2 in human trophoblasts, and demonstrated a mechanism underlying the insufficient trophoblast syncytialization in preeclampsia under hypoxic stress.A positive feedback loop between miR-210 and HIF-1 alpha that is mediated by CPEB2 in human trophoblast demonstrates the mechanism of insufficient syncytialization in preeclampsia under hypoxic stress.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12749
专题	科研部门_疾病机理遗传学和进化医学学科组（姚永刚）
推荐引用方式 GB/T 7714	Wang, Hao,Zhao, Yangyu,Luo, Rongcan,et al. A positive feedback self-regulatory loop between miR-210 and HIF-1 alpha mediated by CPEB2 is involved in trophoblast syncytialization: implication of trophoblast malfunction in preeclampsia[J]. BIOLOGY OF REPRODUCTION,2020,102(3):560-570.
APA	Wang, Hao.,Zhao, Yangyu.,Luo, Rongcan.,Bian, Xiaotao.,Wang, Yongqing.,...&Wang, Yan-Ling.(2020).A positive feedback self-regulatory loop between miR-210 and HIF-1 alpha mediated by CPEB2 is involved in trophoblast syncytialization: implication of trophoblast malfunction in preeclampsia.BIOLOGY OF REPRODUCTION,102(3),560-570.
MLA	Wang, Hao,et al."A positive feedback self-regulatory loop between miR-210 and HIF-1 alpha mediated by CPEB2 is involved in trophoblast syncytialization: implication of trophoblast malfunction in preeclampsia".BIOLOGY OF REPRODUCTION 102.3(2020):560-570.

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