Establishment and transcriptomic features of an immortalized hepatic cell line of the Chinese tree shrew

KIZ OpenIR > 科研部门 > 疾病机理遗传学和进化医学学科组（姚永刚）

	Establishment and transcriptomic features of an immortalized hepatic cell line of the Chinese tree shrew
	Zhang, Xuemei; Yu, Dandan; Wu, Yong; Gu, Tianle; Ma, Na; Dong, Shaozhong; Yao, Yong-Gang
	2020
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
卷号	104 期号:20 页码:8813-8823
摘要	Background The Chinese tree shrew (Tupaia belangeri chinesis) is a rising experimental animal and has been used for studying a variety of human diseases, such as metabolic and viral infectious diseases. Methods In this study, we established an immortalized tree shrew hepatic cell line, ITH6.1, by introducing the simian virus 40 large T antigen gene into primary tree shrew hepatocytes (PTHs). Results The ITH6.1 cell line had a stable cell morphology and proliferation activity. This cell line could be infected by enterovirus 71 (EV71), but not hepatitis C virus (HCV), although the known HCV entry factors, including CD81, SR-BI, CLDN1 and OCLN, were all expressed in the PTHs and ITH6.1 of different passages. Comparison of the transcriptomic features of the PTHs and different passages of the ITH6.1 cells revealed the dynamic gene expression profiles during the transformation. We found that the DNA replication- and cell cycle-related genes were upregulated, whereas the metabolic pathway-related genes were downregulated in early passages of immortalized hepatocytes compared to the PTHs. Furthermore, expression of hepatocytes function-related genes were repressed in ITH6.1 compared to that of PTHs. Conclusion We believe these cellular expression alterations might cause the resistance of the ITH6.1 cell to HCV infection. This tree shrew liver cell line may be a good resource for the field.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12752
专题	科研部门_疾病机理遗传学和进化医学学科组（姚永刚）
推荐引用方式 GB/T 7714	Zhang, Xuemei,Yu, Dandan,Wu, Yong,et al. Establishment and transcriptomic features of an immortalized hepatic cell line of the Chinese tree shrew[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(20):8813-8823.
APA	Zhang, Xuemei.,Yu, Dandan.,Wu, Yong.,Gu, Tianle.,Ma, Na.,...&Yao, Yong-Gang.(2020).Establishment and transcriptomic features of an immortalized hepatic cell line of the Chinese tree shrew.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(20),8813-8823.
MLA	Zhang, Xuemei,et al."Establishment and transcriptomic features of an immortalized hepatic cell line of the Chinese tree shrew".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.20(2020):8813-8823.

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