Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration

	Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration
	Shao, Yong; Wang, Xiao-Bo; Zhang, Jin-Jin; Li, Ming-Li; Wu, Shou-Song; Ma, Xi-Yao; Wang, Xue; Zhao, Hui-Fang; Li, Yuan; Zhu, Helen He; Irwin, David M.; Wang, De-Peng; Zhang, Guo-Jie; Ruan, Jue; Wu, Dong-Dong
	2020
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	11 期号:1
摘要	The earthworm is particularly fascinating to biologists because of its strong regenerative capacity. However, many aspects of its regeneration in nature remain elusive. Here we report chromosome-level genome, large-scale transcriptome and single-cell RNA-sequencing data during earthworm (Eisenia andrei) regeneration. We observe expansion of LINE2 transposable elements and gene families functionally related to regeneration (for example, EGFR, epidermal growth factor receptor) particularly for genes exhibiting differential expression during earthworm regeneration. Temporal gene expression trajectories identify transcriptional regulatory factors that are potentially crucial for initiating cell proliferation and differentiation during regeneration. Furthermore, early growth response genes related to regeneration are transcriptionally activated in both the earthworm and planarian. Meanwhile, single-cell RNA-sequencing provides insight into the regenerative process at a cellular level and finds that the largest proportion of cells present during regeneration are stem cells. The mechanisms regulating regeneration of the earthworm are unclear. Here, the authors use genomic and transcriptomic analysis of the earthworm Eisenia andrei together with Hi-C analysis to identify genes involved and show activation of LINE2 transposable elements on regeneration.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12796
专题	科研部门_进化与发育转录组学（吴东东）
推荐引用方式 GB/T 7714	Shao, Yong,Wang, Xiao-Bo,Zhang, Jin-Jin,et al. Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration[J]. NATURE COMMUNICATIONS,2020,11(1).
APA	Shao, Yong.,Wang, Xiao-Bo.,Zhang, Jin-Jin.,Li, Ming-Li.,Wu, Shou-Song.,...&Wu, Dong-Dong.(2020).Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration.NATURE COMMUNICATIONS,11(1).
MLA	Shao, Yong,et al."Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration".NATURE COMMUNICATIONS 11.1(2020).

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