Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa

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	Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
	Chen, Haitao; Zeng, Yan; Yang, Yongzhi; Huang, Lingli; Tang, Bolin; Zhang, He; Hao, Fei; Li, Wei; Li, Youhan; Liu, Yanbin; Zhang, Xiaoshuang; Zhang, Ru; Zhang, Yesheng; Li, Yongxin; Wang, Kun; He, Hua; Wang, Zhongkai; Fan, Guangyi; Yang, Hui; Bao, Aike; Shang, Zhanhuan; Chen, Jianghua; Wang, Wen; Qiu, Qiang
	2020
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	11 期号:1
摘要	Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy and self-incompatibility. Here, we generate an allele-aware chromosome-level genome assembly for the cultivated alfalfa consisting of 32 allelic chromosomes by integrating high-fidelity single-molecule sequencing and Hi-C data. We further establish an efficient CRISPR/Cas9-based genome editing protocol on the basis of this genome assembly and precisely introduce tetra-allelic mutations into null mutants that display obvious phenotype changes. The mutated alleles and phenotypes of null mutants can be stably inherited in generations in a transgene-free manner by cross pollination, which may help in bypassing the debate about transgenic plants. The presented genome and CRISPR/Cas9-based transgene-free genome editing protocol provide key foundations for accelerating research and molecular breeding of this important forage crop.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12814
专题	基因起源组
推荐引用方式 GB/T 7714	Chen, Haitao,Zeng, Yan,Yang, Yongzhi,et al. Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa[J]. NATURE COMMUNICATIONS,2020,11(1).
APA	Chen, Haitao.,Zeng, Yan.,Yang, Yongzhi.,Huang, Lingli.,Tang, Bolin.,...&Qiu, Qiang.(2020).Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa.NATURE COMMUNICATIONS,11(1).
MLA	Chen, Haitao,et al."Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa".NATURE COMMUNICATIONS 11.1(2020).

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2021102511.pdf（3937KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文