Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

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	Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies
	Zhang, Ru; He, Jinwu; Dong, Zhiwei; Liu, Guichun; Yin, Yuan; Zhang, Xinying; Li, Qi; Ren, Yandong; Yang, Yongzhi; Liu, Wei; Chen, Xianqing; Xia, Wenhao; Duan, Kang; Hao, Fei; Lin, Zeshan; Yang, Jie; Chang, Zhou; Zhao, Ruoping; Wan, Wenting; Lu, Sihan; Peng, Yanqiong; Ge, Siqin; Wang, Wen; Li, Xueyan
	2020
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	10 期号:1
摘要	Fireflies are among the most charismatic insects for their spectacular bioluminescence, but the origin and evolution of bioluminescence remain elusive. Especially, the genic basis of luciferin (D-luciferin) biosynthesis and light patterns is largely unknown. Here, we present the high-quality reference genomes of two fireflies Lamprigera yunnana (1053 Mb) and Abscondita terminalis (501 Mb) with great differences in both morphology and luminous behavior. We sequenced the transcriptomes and proteomes of luminous organs of two species. We created the CRISPR/Cas9-induced mutants of Abdominal B gene without luminous organs in the larvae of A. terminalis and sequenced the transcriptomes of mutants and wild-types. Combining gene expression analyses with comparative genomics, we propose a more complete luciferin synthesis pathway, and confirm the convergent evolution of bioluminescence in insects. Using experiments, the function of the firefly acyl-CoA thioesterase (ACOT1) to convert L-luciferin to D-luciferin was validated for the first time. Comparisons of three-dimension reconstruction of luminous organs and their differentially expressed genes among two species suggest that two positive genes in the calcium signaling pathway and structural difference of luminous organs may play an important role in the evolution of flash pattern. Altogether, our results provide important resources for further exploring bioluminescence in insects.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12816
专题	基因起源组
推荐引用方式 GB/T 7714	Zhang, Ru,He, Jinwu,Dong, Zhiwei,et al. Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies[J]. SCIENTIFIC REPORTS,2020,10(1).
APA	Zhang, Ru.,He, Jinwu.,Dong, Zhiwei.,Liu, Guichun.,Yin, Yuan.,...&Li, Xueyan.(2020).Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies.SCIENTIFIC REPORTS,10(1).
MLA	Zhang, Ru,et al."Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies".SCIENTIFIC REPORTS 10.1(2020).

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