Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

	Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo
	Kabir, Syed Rashel; Dai, Zhi; Nurujjaman, M.; Cui, Xiaoyue; Asaduzzaman, A. K. M.; Sun, Bin; Zhang, Xianning; Dai, Hongjuan; Zhao, Xudong
	2020
发表期刊	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
ISSN	1582-1838
卷号	24 期号:22 页码:13223-13234
摘要	The importance of biogenic silver/silver chloride nanoparticles has become increasing day by day. In the present study, silver/silver chloride nanoparticles (Ag/AgCl-NPs) were synthesized fromKaempferia rotundatuberous rhizome extract to evaluate the antiproliferative activity against human glioblastoma stem cells (GSCs) in vitro and Ehrlich ascites carcinoma (EAC) cells in vivo in mice. Synthesis of nanoparticles was confirmed by colour change and UV-visible spectrum and characterized by TEM, XRD, TGA, AFM and FTIR.K rotundaand recently synthesizedZizyphus mauritianafruit extract-mediated Ag/AgCl-NPs inhibited 77.2% and 71% of GSCs growth at 32 mu g/mL concentration with the IC(50)values of 6.8 and 10.4 mu g/mL, respectively. Cell morphological studies and caspase-3 immunofluorescence assay revealed that both biogenic nanoparticles induced apoptosis in GSCs. Expression levels of several genes were checked by real-time PCR after treatment withK rotundatuberous rhizome-mediated Ag/AgCl-NPs. PARP, EGFR, NOTCH2 and STAT3 gene expression were decreased with the increase of NF kappa B, TLR9, IL1, TNF alpha, IKK and p21 gene that would be the cause of induction of apoptosis in GSCs. The cell cycle arrest at G(2)/M phase was confirmed by flow cytometric assay. Both nanoparticles were injected intraperitoneally to rapidly growing EAC cells for 5 consecutive days. Approximately, 32.3% and 55% EAC cells growth were inhibited byK rotundatuberous rhizome-mediated Ag/AgCl-NPs at 6 and 12 mg/kg/day doses, respectively while only 20% cell growth inhibition was monitored at 12 mg/kg/day dose ofZ mauritiana-mediated Ag/AgCl-NPs. From the above results, it can be concluded that presently synthesized nanoparticles would be a potent anticancer agent.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12957
专题	科研部门_进化发育生物学（刘振）
推荐引用方式 GB/T 7714	Kabir, Syed Rashel,Dai, Zhi,Nurujjaman, M.,et al. Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2020,24(22):13223-13234.
APA	Kabir, Syed Rashel.,Dai, Zhi.,Nurujjaman, M..,Cui, Xiaoyue.,Asaduzzaman, A. K. M..,...&Zhao, Xudong.(2020).Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,24(22),13223-13234.
MLA	Kabir, Syed Rashel,et al."Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 24.22(2020):13223-13234.

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