Shifting up a gear with iDNA: From mammal detection events to standardised surveys

	Shifting up a gear with iDNA: From mammal detection events to standardised surveys
	Abrams, JF; Horig, LA; Brozovic, R; Axtner, J; Crampton-Platt, A; Mohamed, A; Wong, ST; Sollmann, R; Yu, DW; Wilting, A
	2019
发表期刊	JOURNAL OF APPLIED ECOLOGY
ISSN	0021-8901
卷号	56 期号:7 页码:1637-1648
摘要	Invertebrate-derived DNA (iDNA), in combination with high throughput sequencing, has been proposed as a cost-efficient and powerful tool to survey vertebrate species. Previous studies, however, have only provided evidence that vertebrates can be detected using iDNA, but have not taken the next step of placing these detection events within a statistical framework that allows for robust biodiversity assessments. Here, we compare concurrent iDNA and camera-trap surveys. Leeches were repeatedly collected in close vicinity to 64 camera-trap stations in Sabah, Malaysian Borneo. We analyse iDNA-derived mammalian detection events in a modern occupancy model that accounts for imperfect detection and compare the results with those from occupancy models parameterised with camera-trap-derived detection events. We also combine leech-iDNA and camera-trap data in a single occupancy model. We found consistent estimates of occupancy probabilities produced by our camera-trap and leech datasets. This indicates that the metabarcoding of leech-iDNA method provides reasonable estimates of occupancy and may be a suitable method for studying and monitoring mammal species in tropical rainforests. However, we also show that a more extensive collection of leeches would be needed to assess mammal biodiversity with a robustness similar to that of camera traps. As certain taxa were only detected in leeches, we see great potential in complementing camera-trap studies with the iDNA approach, as long as the collection of leeches follows a robust and standardised sampling scheme. Synthesis and applications. Here, we describe an approach to analyse detection records of mammals derived from leech samples using an occupancy framework that accounts for leech-specific factors influencing the detection probability. We further combined camera trap and leech data, which lead to increased confidence in occupancy estimates. Our approach is not restricted to the processing of leech samples, but can be used for the analysis of other invertebrate DNA and environmental DNA data. Our study is the first step to shift the application of invertebrate DNA studies from opportunistic ad-hoc collections to the systematic surveys required for long-term management of wildlife populations.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12994
专题	科研部门_生态学与环境保护中心（俞维理）
推荐引用方式 GB/T 7714	Abrams, JF,Horig, LA,Brozovic, R,et al. Shifting up a gear with iDNA: From mammal detection events to standardised surveys[J]. JOURNAL OF APPLIED ECOLOGY,2019,56(7):1637-1648.
APA	Abrams, JF.,Horig, LA.,Brozovic, R.,Axtner, J.,Crampton-Platt, A.,...&Wilting, A.(2019).Shifting up a gear with iDNA: From mammal detection events to standardised surveys.JOURNAL OF APPLIED ECOLOGY,56(7),1637-1648.
MLA	Abrams, JF,et al."Shifting up a gear with iDNA: From mammal detection events to standardised surveys".JOURNAL OF APPLIED ECOLOGY 56.7(2019):1637-1648.

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2020101314.pdf（1915KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文