Mutual editing of alternative splicing between breast cancer cells and macrophages

	Mutual editing of alternative splicing between breast cancer cells and macrophages
	Ding, WB; Li, DD; Zhang, PX; Shi, L; Dai, H; Li, Y; Bao, X; Wang, Y; Zhang, HL; Deng, L
	2019
发表期刊	ONCOLOGY REPORTS
ISSN	1021-335X
卷号	42 期号:2 页码:629-656
摘要	Breast cancer is a highly heterogeneous disease and numerous secreted factors may differentially contribute to a macrophage phenotype whose extensive infiltration is generally regarded as indicative of an unfavorable outcome. How different breast tumor cells and macrophage cells interplay or influence each other on the alternative splicing (AS) level have not been characterized. Here, we exploited one previous study, which investigated the interplay between macrophages and estrogen receptor-positive (ER+) breast cancer and triple-negative breast cancer (TNBC) at the transcriptional level, to investigate the tumor-macrophage crosstalk at the AS level. In the present study, it was demonstrated that biological processes such as DNA damage and DNA repair were significantly affected both in ER+ breast cancer and TNBC by co-culturing with macrophages, whereas biological pathways altered in macrophages co-cultured with tumor cells depended on the breast cancer type. Specifically, biological processes altered in macrophages co-cultured with ER+ breast cancer were enriched in RNA processing and translation-related pathways whereas biological processes altered in macrophages co-cultured with TNBC were mainly enriched in protein transport pathways. We also analyzed the sequence features of skip exons among different conditions. In addition, putative splicing factors which were responsible for the altered AS profile in each condition were identified. The findings of the present study revealed significant tumor-macrophage crosstalk at the AS level which may facilitate the development of new therapeutic strategies for cancer.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13014
专题	科研部门_发育的印迹调控与进化学（焦保卫）
推荐引用方式 GB/T 7714	Ding, WB,Li, DD,Zhang, PX,et al. Mutual editing of alternative splicing between breast cancer cells and macrophages[J]. ONCOLOGY REPORTS,2019,42(2):629-656.
APA	Ding, WB.,Li, DD.,Zhang, PX.,Shi, L.,Dai, H.,...&Deng, L.(2019).Mutual editing of alternative splicing between breast cancer cells and macrophages.ONCOLOGY REPORTS,42(2),629-656.
MLA	Ding, WB,et al."Mutual editing of alternative splicing between breast cancer cells and macrophages".ONCOLOGY REPORTS 42.2(2019):629-656.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2020101330.pdf（1694KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文