Endogenous pore-forming protein complex targets acidic glycosphingolipids in lipid rafts to initiate endolysosome regulation

	Endogenous pore-forming protein complex targets acidic glycosphingolipids in lipid rafts to initiate endolysosome regulation
	Guo, XL; Liu, LZ; Wang, QQ; Liang, JY; Lee, WH; Xiang, Y; Li, SA; Zhang, Y
	2019
发表期刊	COMMUNICATIONS BIOLOGY
卷号	2 页码:-
摘要	Bacterial pore-forming toxin aerolysin-like proteins (ALPs) are widely distributed in animals and plants. However, functional studies on these ALPs remain in their infancy. beta gamma-CAT is the first example of a secreted pore-forming protein that functions to modulate the endolysosome pathway via endocytosis and pore formation on endolysosomes. However, the specific cell surface molecules mediating the action of beta gamma-CAT remain elusive. Here, the actions of beta gamma-CAT were largely attenuated by either addition or elimination of acidic glycosphingolipids (AGSLs). Further study revealed that the ALP and trefoil factor (TFF) subunits of beta gamma-CAT bind to gangliosides and sulfatides, respectively. Additionally, disruption of lipid rafts largely impaired the actions of beta gamma-CAT. Finally, the ability of beta gamma-CAT to clear pathogens was attenuated in AGSL-eliminated frogs. These findings revealed a previously unknown double binding pattern of an animal-secreted ALP in complex with TFF that initiates ALP-induced endolysosomal pathway regulation, ultimately leading to effective antimicrobial responses.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13097
专题	科研部门_生物毒素与人类疾病（张云）
推荐引用方式 GB/T 7714	Guo, XL,Liu, LZ,Wang, QQ,et al. Endogenous pore-forming protein complex targets acidic glycosphingolipids in lipid rafts to initiate endolysosome regulation[J]. COMMUNICATIONS BIOLOGY,2019,2:-.
APA	Guo, XL.,Liu, LZ.,Wang, QQ.,Liang, JY.,Lee, WH.,...&Zhang, Y.(2019).Endogenous pore-forming protein complex targets acidic glycosphingolipids in lipid rafts to initiate endolysosome regulation.COMMUNICATIONS BIOLOGY,2,-.
MLA	Guo, XL,et al."Endogenous pore-forming protein complex targets acidic glycosphingolipids in lipid rafts to initiate endolysosome regulation".COMMUNICATIONS BIOLOGY 2(2019):-.

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