Dynamic global analysis of transcription reveals the role of miRNAs in synergistic stabilization of gene expression

	Dynamic global analysis of transcription reveals the role of miRNAs in synergistic stabilization of gene expression
	Ma, FQ; Lu, GA; Chen, QJ; Ruan, YS; Li, X; Lu, XM; Li, CY
	2020
发表期刊	SCIENCE BULLETIN
ISSN	2095-9273
卷号	65 期号:24 页码:2130-2140
摘要	Buffering exogenous perturbation is crucial to maintain transcriptional homeostasis during development. While miRNAs have been speculated to play a role in stability maintenance, previous studies seeking to check this conjecture focused on measurements of transcript levels at steady state or involved individual miRNA targets. We measured whole-genome expression dynamics by introducing a transient perturbation and establishing a perturbation and recovery system in Drosophila larvae. We inhibited all transcription and assayed transcriptomes at several time points during recovery from inhibition. We performed these experiments in the wild type and miRNA-deficient genetic backgrounds. Consistent with theories about miRNAs' function in stabilizing the transcriptome, we find that attenuating miRNA expression leads to weak impairment in degradation of targets but strong destabilization of target genes when transcription is re-activated. We further fitted a model that captures the essential aspects of transcription dynamics in our experiments and found that the miRNA target transcripts uniformly overshoot the original steady state as they recover from a general inhibition of transcription if global miRNA levels are reduced. Collectively, our results provide experimental evidence for the idea that miRNAs act cumulatively to stabilize the transcriptional regulatory network. We therefore found a promising approach to assess the effect of these molecules on transcription dynamics. (c) 2020 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13147
专题	科研部门_适应性进化与进化医学（吕雪梅）昆明动物研究所
推荐引用方式 GB/T 7714	Ma, FQ,Lu, GA,Chen, QJ,et al. Dynamic global analysis of transcription reveals the role of miRNAs in synergistic stabilization of gene expression[J]. SCIENCE BULLETIN,2020,65(24):2130-2140.
APA	Ma, FQ.,Lu, GA.,Chen, QJ.,Ruan, YS.,Li, X.,...&Li, CY.(2020).Dynamic global analysis of transcription reveals the role of miRNAs in synergistic stabilization of gene expression.SCIENCE BULLETIN,65(24),2130-2140.
MLA	Ma, FQ,et al."Dynamic global analysis of transcription reveals the role of miRNAs in synergistic stabilization of gene expression".SCIENCE BULLETIN 65.24(2020):2130-2140.

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