A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis

	A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis
	Guo, XP; Li, BW; Liang, SH; Lai, R; Liu, H
	2020
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	528 期号:1 页码:99-104
摘要	A novel Kunitz-type neurotoxin peptide that inhibited voltage-gated sodium channel was purified and characterized from the skin secretions of rufous-spotted torrent frog, Amolops loloensis. It has a 240-bp cDNA encoding an 79-amino acid residue (aa) precursor protein containing 6 half-cysteines. The precursor was proven to release a 57-aa mature peptide with amino acid sequence, DRNPICNLPPKEGFCLWMMRRSFFNPSKGRCDTFGYRGCGGNKNNFETPRACKEACG. The mature was named amotoxin. Amotoxin shares sequence homology with other Kunitz-type toxins and also has three cysteine bridges. Amotoxin showed an inhibitory ability against trypsin with an inhibitory constant (Ki) of 0.087 mu M. To the best of our knowledge, this is the first gene-encoded neurotoxin found in Amolops loloensis. Recombinant amotoxin showed similar functional properties as the native amotoxin. The functional properties of amotoxin may provide insights into the ecological adaptation of amphibians and deepen our understanding about the biological function spectrum of amphibian skin peptides. (C) 2020 Elsevier Inc. All rights reserved.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13235
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
推荐引用方式 GB/T 7714	Guo, XP,Li, BW,Liang, SH,et al. A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,528(1):99-104.
APA	Guo, XP,Li, BW,Liang, SH,Lai, R,&Liu, H.(2020).A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,528(1),99-104.
MLA	Guo, XP,et al."A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 528.1(2020):99-104.

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