Competition-based screening helps to secure the evolutionary stability of a defensive microbiome

	Competition-based screening helps to secure the evolutionary stability of a defensive microbiome
	Worsley, SF; Innocent, TM; Holmes, NA; Al-Bassam, MM; Schiott, M; Wilkinson, B; Murrell, JC; Boomsma, JJ; Yu, DW; Hutchings, MI
	2021
发表期刊	BMC BIOLOGY
卷号	19 期号:1
摘要	Background The cuticular microbiomes of Acromyrmex leaf-cutting ants pose a conundrum in microbiome biology because they are freely colonisable, and yet the prevalence of the vertically transmitted bacteria Pseudonocardia, which contributes to the control of Escovopsis fungus garden disease, is never compromised by the secondary acquisition of other bacterial strains. Game theory suggests that competition-based screening can allow the selective recruitment of antibiotic-producing bacteria from the environment, by providing abundant resources to foment interference competition between bacterial species and by using Pseudonocardia to bias the outcome of competition in favour of antibiotic producers. Results Here, we use RNA-stable isotope probing (RNA-SIP) to confirm that Acromyrmex ants can maintain a range of microbial symbionts on their cuticle by supplying public resources. We then used RNA sequencing, bioassays, and competition experiments to show that vertically transmitted Pseudonocardia strains produce antibacterials that differentially reduce the growth rates of other microbes, ultimately biassing the bacterial competition to allow the selective establishment of secondary antibiotic-producing strains while excluding non-antibiotic-producing strains that would parasitise the symbiosis. Conclusions Our findings are consistent with the hypothesis that competition-based screening is a plausible mechanism for maintaining the integrity of the co-adapted mutualism between the leaf-cutting ant farming symbiosis and its defensive microbiome. Our results have broader implications for explaining the stability of other complex symbioses involving horizontal acquisition.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13262
专题	科研部门_生态学与环境保护中心（俞维理）
推荐引用方式 GB/T 7714	Worsley, SF,Innocent, TM,Holmes, NA,et al. Competition-based screening helps to secure the evolutionary stability of a defensive microbiome[J]. BMC BIOLOGY,2021,19(1).
APA	Worsley, SF.,Innocent, TM.,Holmes, NA.,Al-Bassam, MM.,Schiott, M.,...&Hutchings, MI.(2021).Competition-based screening helps to secure the evolutionary stability of a defensive microbiome.BMC BIOLOGY,19(1).
MLA	Worsley, SF,et al."Competition-based screening helps to secure the evolutionary stability of a defensive microbiome".BMC BIOLOGY 19.1(2021).

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