| High-throughput mapping of a whole rhesus monkey brain at micrometer resolution |
| Xu, F; Shen, Y; Ding, LF; Yang, CY; Tan, H; Wang, H; Zhu, QY; Xu, R; Wu, FY; Xiao, YY; Xu, C; Li, QW; Su, P; Zhang, LI; Dong, HW; Desimone, R; Xu, FQ; Hu, XT; Lau, PM; Bi, GQ
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| 2021
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| 发表期刊 | NATURE BIOTECHNOLOGY
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| ISSN | 1087-0156
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| 卷号 | 39期号:12页码:1521-+ |
| 摘要 | Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and the relatively low throughput of available microscopy methods. Here, we present an approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed fluorescence microscopy implementing improved volumetric imaging with synchronized on-the-fly-scan and readout technique, and is capable of completing whole-brain imaging of a rhesus monkey at 1 x 1x 2.5 mu m(3) voxel resolution within 100 h. We also developed a highly efficient method for long-range tracing of sparse axonal fibers in datasets numbering hundreds of terabytes. This pipeline, which we call serial sectioning and clearing, three-dimensional microscopy with semi-automated reconstruction and tracing (SMART), enables effective connectome-scale mapping of large primate brains. With SMART, we were able to construct a cortical projection map of the mediodorsal nucleus of the thalamus and identify distinct turning and routing patterns of individual axons in the cortical folds while approaching their arborization destinations. |
| 收录类别 | sci
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13276
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| 专题 | 科研部门_神经系统编码（胡新天）
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推荐引用方式
GB/T 7714 |
Xu, F,Shen, Y,Ding, LF,et al. High-throughput mapping of a whole rhesus monkey brain at micrometer resolution[J]. NATURE BIOTECHNOLOGY,2021,39(12):1521-+.
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| APA |
Xu, F.,Shen, Y.,Ding, LF.,Yang, CY.,Tan, H.,...&Bi, GQ.(2021).High-throughput mapping of a whole rhesus monkey brain at micrometer resolution.NATURE BIOTECHNOLOGY,39(12),1521-+.
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| MLA |
Xu, F,et al."High-throughput mapping of a whole rhesus monkey brain at micrometer resolution".NATURE BIOTECHNOLOGY 39.12(2021):1521-+.
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