Transcriptome innovations in primates revealed by single-molecule long-read sequencing

	Transcriptome innovations in primates revealed by single-molecule long-read sequencing
	Ferrandez-Peral, L; Zhan, XY; Alvarez-Estape, M; Chiva, C; Esteller-Cucala, P; Garcia-Perez, R; Julia, E; Lizano, E; Fornas, O; Sabido, E; Li, QY; Marques-Bonet, T; Juan, D; Zhang, GJ
	2022
发表期刊	GENOME RESEARCH
ISSN	1088-9051
卷号	32 期号:8 页码:1448-1462
摘要	Transcriptomic diversity greatly contributes to the fundamentals of disease, lineage-specific biology, and environmental adaptation. However, much of the actual isoform repertoire contributing to shaping primate evolution remains unknown. Here, we combined deep long- and short-read sequencing complemented with mass spectrometry proteomics in a panel of lymphoblastoid cell lines (LCLs) from human, three other great apes, and rhesus macaque, producing the largest full-length isoform catalog in primates to date. Around half of the captured isoforms are not annotated in their reference genomes, significantly expanding the gene models in primates. Furthermore, our comparative analyses unveil hundreds of transcriptomic innovations and isoform usage changes related to immune function and immunological disorders. The confluence of these evolutionary innovations with signals of positive selection and their limited impact in the proteome points to changes in alternative splicing in genes involved in immune response as an important target of recent regulatory divergence in primates.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13442
专题	科研部门_遗传多样性基因组学研究组（张国捷）
推荐引用方式 GB/T 7714	Ferrandez-Peral, L,Zhan, XY,Alvarez-Estape, M,et al. Transcriptome innovations in primates revealed by single-molecule long-read sequencing[J]. GENOME RESEARCH,2022,32(8):1448-1462.
APA	Ferrandez-Peral, L.,Zhan, XY.,Alvarez-Estape, M.,Chiva, C.,Esteller-Cucala, P.,...&Zhang, GJ.(2022).Transcriptome innovations in primates revealed by single-molecule long-read sequencing.GENOME RESEARCH,32(8),1448-1462.
MLA	Ferrandez-Peral, L,et al."Transcriptome innovations in primates revealed by single-molecule long-read sequencing".GENOME RESEARCH 32.8(2022):1448-1462.

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2023031540.pdf（5155KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文