| A combination vaccine against SARS-CoV-2 and H1N1 influenza based on receptor binding domain trimerized by six-helix bundle fusion core |
| Shi, R; Zeng, JW; Xu, L ; Wang, FZ; Duan, XM; Wang, Y; Wu, Z; Yu, DD; Huang, QR; Yao, YG; Yan, JH
|
| 2022
|
| 发表期刊 | EBIOMEDICINE
 |
| ISSN | 2352-3964
|
| 卷号 | 85 |
| 摘要 | Background Increasing severe morbidity and mortality by simultaneous or sequential infections with SARS-CoV-2 and influenza A viruses (IAV), especially in the elderly and obese patients, highlight the urgency of developing a combination vaccine against COVID-19 and influenza.Methods Self-assembling SARS-CoV-2 RBD-trimer and Influenza H1N1 HA1-trimer antigens were constructed, upon the stable fusion core in post-fusion conformation. Immunogenicity of SARS-CoV-2 RBD-trimer vaccine and H1N1 HA1-trimer antigens candidates were evaluated in mice. Protection efficacy of a combination vaccine candidate against SARS-CoV-2 and IAV challenge was identified using the K18-hACE2 mouse model.Findings Both the resultant RBD-trimer for SARS-CoV-2 and HA1-trimer for H1N1 influenza fully exposed receptor-binding motifs (RBM) or receptor-binding site (RBS). Two-dose RBD-trimer induced significantly higher binding and neutralizing antibody titers, and also a strong Th1/Th2 balanced cellular immune response in mice. Similarly, the HA1-trimer vaccine was confirmed to exhibit potent immunogenicity in mice. A combination vaccine candidate, composed of RBD-trimer and HA1-trimer, afforded high protection efficacy in mouse models against stringent lethal SARS-CoV-2 and homogenous H1N1 influenza co-infection, characterized by 100% survival rate.Interpretation Our results represent a proof of concept for a combined vaccine candidate based on trimerized recep-tor binding domain against co-epidemics of COVID-19 and influenza.Copyright (c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) |
| 收录类别 | sci
|
| 语种 | 英语
|
| 文献类型 | 期刊论文
|
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13487
|
| 专题 | 科研部门_疾病机理遗传学和进化医学学科组（姚永刚）
|
推荐引用方式
GB/T 7714 |
Shi, R,Zeng, JW,Xu, L,et al. A combination vaccine against SARS-CoV-2 and H1N1 influenza based on receptor binding domain trimerized by six-helix bundle fusion core[J]. EBIOMEDICINE,2022,85.
|
| APA |
Shi, R.,Zeng, JW.,Xu, L.,Wang, FZ.,Duan, XM.,...&Yan, JH.(2022).A combination vaccine against SARS-CoV-2 and H1N1 influenza based on receptor binding domain trimerized by six-helix bundle fusion core.EBIOMEDICINE,85.
|
| MLA |
Shi, R,et al."A combination vaccine against SARS-CoV-2 and H1N1 influenza based on receptor binding domain trimerized by six-helix bundle fusion core".EBIOMEDICINE 85(2022).
|
修改评论