Identification and functional characteristics of two TLR5 subtypes in S. grahami

	Identification and functional characteristics of two TLR5 subtypes in S. grahami
	Yang, SY; Leng, SZ; Li, YK; Wang, XA; Zhang, YW; Wu, AL; Gao, YF; Wu, JY; Zeng, XY; Du, XG; Pan, XF
	2022
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	131 页码:707-717
摘要	TLR5, as a member of Toll-like receptors (TLRs) family in mammals, is responsible for recognizing bacterial flagellin and initiating innate immunity, but its function is still unclear in fish species. In this study, two family members of TLR5 were cloned and identified from Sinocyclocheilus grahami (S. grahami), named sgTLR5a and sgTLR5b. The length of coding sequence of sgTLR5a and sgTLR5b is 2,622 bp and 2,658 bp, encoding 873 and 885 amino acids, respectively. Molecular phylogenetic analysis indicates that sgTLR5a and sgTLR5b have the closest genetic relationship with TLR5M (membrane-type) of Cyprinus carpio and Schizothorax prenanti, respectively. sgTLR5a and sgTLR5b were widely expressed in various tested tissues, of which the expression levels were the highest in skin tissue. After stimulations of Aeromonas hydrophila (A. hydrophila) and flagellin, the expression levels of sgTLR5a and sgTLR5b in liver, spleen and head kidney tissues were strongly up-regulated, but LPS stimulation only increased the expression of sgTLR5b in these tissues. The luciferase reporter assay displayed that sgTLR5a and sgTLR5b could specifically recognize bacterial flagellin and A. hydrophila and activate the down-stream NF-Kappa B signaling pathway in HEK293T cells. Moreover, the overexpression of sgTLR5a and sgTLR5b in EPC cells up-regulated the expression levels of IL-8 and TNF. sgTLR5a and sgTLR5b were observed to locate in the intracellular region by confocal microscope. Interestingly, we found that the NF-Kappa B signaling pathway was positively regulated by co-transfecting sgTLR5a or sgTLR5b with TLR trafficking chaperone sgUNC93B1. In conclusion, our results reveal sgTLR5a and sgTLR5b may play an important role in antibacterial response by activating the NF-Kappa B signaling pathway.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13625
专题	科研部门_系统进化与生物地理学（杨君兴）
推荐引用方式 GB/T 7714	Yang, SY,Leng, SZ,Li, YK,et al. Identification and functional characteristics of two TLR5 subtypes in S. grahami[J]. FISH & SHELLFISH IMMUNOLOGY,2022,131:707-717.
APA	Yang, SY.,Leng, SZ.,Li, YK.,Wang, XA.,Zhang, YW.,...&Pan, XF.(2022).Identification and functional characteristics of two TLR5 subtypes in S. grahami.FISH & SHELLFISH IMMUNOLOGY,131,707-717.
MLA	Yang, SY,et al."Identification and functional characteristics of two TLR5 subtypes in S. grahami".FISH & SHELLFISH IMMUNOLOGY 131(2022):707-717.

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