Single-cell profiling of long noncodingRNAsand their cell lineage commitment roles viaRNA-DNA-DNAtriplex formation in mammary epithelium

	Single-cell profiling of long noncodingRNAsand their cell lineage commitment roles viaRNA-DNA-DNAtriplex formation in mammary epithelium
	Xu, HB; Yang, X; Huang, WR; Ma, YJ; Ke, H; Zou, L; Yang, Q; Jiao, BW
	2020
发表期刊	STEM CELLS
ISSN	1066-5099
卷号	38 期号:12 页码:1594-1611
摘要	Long noncoding RNAs (lncRNAs), which are crucial for organ development, exhibit cell-specific expression. Thus, transcriptomic analysis based on total tissue (bulk-seq) cannot accurately reflect the expression pattern of lncRNAs. Here, we used high-throughput single-cell RNA-seq data to investigate the role of lncRNAs using the hierarchical model of mammary epithelium. With our comprehensive annotation of the mammary epithelium, lncRNAs showed much greater cell-lineage specific expression than coding genes. The lineage-specific lncRNAs were functionally correlated with lineage commitment through the coding genes via thecis- andtrans-effects of lncRNAs. For the working mechanism, lncRNAs formed a triplex structure with the DNA helix to regulate downstream lineage-specific marker genes. We used lncRNA-Carmnas an example to validate the above findings.Carmn, which is specifically expressed in mammary gland stem cells (MaSCs) and basal cells, positively regulated the Wnt signaling ligandWnt10athrough formation of a lncRNA-DNA-DNA triplex, and thus controlled the stemness of MaSCs. Our study suggests that lncRNAs play essential roles in cell-lineage commitment and provides an approach to decipher lncRNA functions based on single-cell RNA-seq data.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13638
专题	科研部门_发育的印迹调控与进化学（焦保卫）
推荐引用方式 GB/T 7714	Xu, HB,Yang, X,Huang, WR,et al. Single-cell profiling of long noncodingRNAsand their cell lineage commitment roles viaRNA-DNA-DNAtriplex formation in mammary epithelium[J]. STEM CELLS,2020,38(12):1594-1611.
APA	Xu, HB.,Yang, X.,Huang, WR.,Ma, YJ.,Ke, H.,...&Jiao, BW.(2020).Single-cell profiling of long noncodingRNAsand their cell lineage commitment roles viaRNA-DNA-DNAtriplex formation in mammary epithelium.STEM CELLS,38(12),1594-1611.
MLA	Xu, HB,et al."Single-cell profiling of long noncodingRNAsand their cell lineage commitment roles viaRNA-DNA-DNAtriplex formation in mammary epithelium".STEM CELLS 38.12(2020):1594-1611.

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