The dynamics of DNA methylation fidelity during mouse embryonic stem cell self-renewal and differentiation

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	The dynamics of DNA methylation fidelity during mouse embryonic stem cell self-renewal and differentiation
	Zhao, L; Sun, MA; Li, ZJ; Bai, X; Yu, M; Wang, M; Liang, LJ; Shao, XJ; Arnovitz, S; Wang, QF; He, C; Lu, XM; Chen, JJ; Xie, HH
	2014
发表期刊	GENOME RESEARCH
ISSN	1088-9051
卷号	24 期号:8 页码:1296-1307
摘要	The faithful transmission of DNA methylation patterns through cell divisions is essential for the daughter cells to retain a proper cell identity. To achieve a comprehensive assessment of methylation fidelity, we implemented a genome-scale hairpin bisulfite sequencing approach to generate methylation data for DNA double strands simultaneously. We show here that methylation fidelity increases globally during differentiation of mouse embryonic stem cells (mESCs), and is particularly high in the promoter regions of actively expressed genes and positively correlated with active histone modification marks and binding of transcription factors. The majority of intermediately (40%-60%) methylated CpG dinucleotides are hemi-methylated and have low methylation fidelity, particularly in the differentiating mESCs. While 5-hmC and 5-mC tend to coexist, there is no significant correlation between 5-hmC levels and methylation fidelity. Our findings may shed new light on our understanding of the origins of methylation variations and the mechanisms underlying DNA methylation transmission.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13659
专题	个人在本单位外知识产出
推荐引用方式 GB/T 7714	Zhao, L,Sun, MA,Li, ZJ,et al. The dynamics of DNA methylation fidelity during mouse embryonic stem cell self-renewal and differentiation[J]. GENOME RESEARCH,2014,24(8):1296-1307.
APA	Zhao, L.,Sun, MA.,Li, ZJ.,Bai, X.,Yu, M.,...&Xie, HH.(2014).The dynamics of DNA methylation fidelity during mouse embryonic stem cell self-renewal and differentiation.GENOME RESEARCH,24(8),1296-1307.
MLA	Zhao, L,et al."The dynamics of DNA methylation fidelity during mouse embryonic stem cell self-renewal and differentiation".GENOME RESEARCH 24.8(2014):1296-1307.

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2023041210.pdf（1730KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文