Human fibroblasts facilitate the generation of iPSCs-derived mammary-like organoids

KIZ OpenIR > 科研部门 > 肿瘤生物学（陈策实）

	Human fibroblasts facilitate the generation of iPSCs-derived mammary-like organoids
	Dai, XQ; Wang, XY; Yang, CY; Huang, MB; Zhou, ZM; Qu, Y; Cui, XJ; Liu, R; Chen, CS
	2022
发表期刊	STEM CELL RESEARCH & THERAPY
卷号	13 期号:1
摘要	Background Breast cancer is the most common malignancy in women worldwide, and its treatment largely depends on mastectomy. Patients after mastectomy suffer from crippled body image, self-esteem, and quality of life. Post-mastectomy breast reconstruction can improve patients' psychosocial health. Although silicone and fat have been widely used for breast reconstruction, they have remarkable limitations. Our study aimed to establish an improved method for breast reconstruction from human-induced pluripotent stem cells (iPSCs). Methods We used a two-step procedure to induce mammary-like organoids (MLOs) from iPSCs and applied transcriptome sequencing to analyze the gene expression profiles during the development process from embryoid bodies (mEBs) to MLOs. Moreover, we evaluated the in vitro effect of fibroblasts cell line HFF (human foreskin fibroblasts) on the size and morphology of MLOs and explored the in vivo effect of HFF on regeneration rate of MLOs. Results MLOs had a similar gene expression profile and morphogenesis as the normal mammary glands. Furthermore, the addition of HFF increases the branching ratio and organoid diameters and facilitates the formation of multiple cell layers duct-like structures in MLOs in vitro. Finally, orthotopical transplantation of the MLOs to cleared mammary gland fad pad of NSG mice showed that HFF increases the formation of mammary gland-like structures. Conclusions Fibroblasts facilitate iPSC-derived MLOs to generate mammary gland-like structures in both in vitro and in vivo conditions. Our findings lay a foundation for breast reconstruction by using iPSCs.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13692
专题	科研部门_肿瘤生物学（陈策实）
推荐引用方式 GB/T 7714	Dai, XQ,Wang, XY,Yang, CY,et al. Human fibroblasts facilitate the generation of iPSCs-derived mammary-like organoids[J]. STEM CELL RESEARCH & THERAPY,2022,13(1).
APA	Dai, XQ.,Wang, XY.,Yang, CY.,Huang, MB.,Zhou, ZM.,...&Chen, CS.(2022).Human fibroblasts facilitate the generation of iPSCs-derived mammary-like organoids.STEM CELL RESEARCH & THERAPY,13(1).
MLA	Dai, XQ,et al."Human fibroblasts facilitate the generation of iPSCs-derived mammary-like organoids".STEM CELL RESEARCH & THERAPY 13.1(2022).

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