| The revised complete mitogenome sequence of the tree frog Polypedates megacephalus (Anura, Rhacophoridae) by next-generation sequencing and phylogenetic analysis |
| Huang, A; Liu, S; Li, HJ; Luo, HD; Ni, QY; Yao, YF; Xu, HL; Zeng, B; Li, Y; Wei, ZM; Li, S; Zhang, MW
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| 2019
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| 发表期刊 | PEERJ
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| ISSN | 2167-8359
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| 卷号 | 7 |
| 摘要 | The mitochondrial genome (mitogenome) sequence of the tree frog Polypedates megacephalus (16,473 bp) was previously reported as having the unusual characteristic of lacking the ND5 gene. In this study, a new mitogenome of P. megacephalus (19,952 bp) was resequenced using the next-generation sequencing (NGS) and standard Sanger sequencing technologies. It was discovered that the ND5 gene was not lost but translocated to the control region (CR) from its canonical location between the ND4 and ND6 genes. In addition, a duplicated control region was found in the new mitogenome of this species. Conservative region identification of the ND5 gene and phylogenetic analysis confirmed that the ND5 gene was located between two control regions. The phylogenetic relationship among 20 related species of anura revealed a rearrangement of the ND5 gene during the evolutionary process. These results also highlighted the advantages of next-generation sequencing. It will not only decrease the time and cost of sequencing, but also will eliminate the errors in published mitogenome databases. |
| 收录类别 | sci
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13750
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| 专题 | 管理、支撑部门_动物博物馆
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推荐引用方式
GB/T 7714 |
Huang, A,Liu, S,Li, HJ,et al. The revised complete mitogenome sequence of the tree frog Polypedates megacephalus (Anura, Rhacophoridae) by next-generation sequencing and phylogenetic analysis[J]. PEERJ,2019,7.
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| APA |
Huang, A.,Liu, S.,Li, HJ.,Luo, HD.,Ni, QY.,...&Zhang, MW.(2019).The revised complete mitogenome sequence of the tree frog Polypedates megacephalus (Anura, Rhacophoridae) by next-generation sequencing and phylogenetic analysis.PEERJ,7.
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| MLA |
Huang, A,et al."The revised complete mitogenome sequence of the tree frog Polypedates megacephalus (Anura, Rhacophoridae) by next-generation sequencing and phylogenetic analysis".PEERJ 7(2019).
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