Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes)

	Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes)
	He, JW; Liu, GC; Dong, PX; Dong, ZW; Zhao, RP; Wang, W; Li, XY
	2021
发表期刊	PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
ISSN	1474-905X
卷号	20 期号:8 页码:1053-1067
摘要	Firefly adult bioluminescence functions as signal communication between sexes. How sympatric sibling species with similar glow pattern recognize their conspecific mates remains largely unknown. To better understand the role of the luciferases of sympatric fireflies in recognizing mates, we cloned the luciferase genes of three sympatric forest dwelling fireflies (Diaphanes nubilus, Diaphanes pectinealis, and Diaphanes sp2) and evaluated their enzyme characteristics. Our data show that the amino acid (AA) sequences of all three luciferases are highly conserved, including the identities (D. nubilus vs D. pectinealis: 99%; D. nubilus vs Diaphanes sp2: 98.5%; D. pectinealis vs Diaphanes sp2: 99.4%) and the protein structures. Three recombinant luciferases produced in vitro all possess significant luminescence activity at pH 7.8, and similar maximum emission spectrum (D. nubilus: 562 nm; D. pectinealis and Diaphanes sp2: 564 nm). They show the highest activity at 10 degrees C (D. pectinealis, Diaphanes sp2) and 15 degrees C (D. nubilus), and completely inactivation at 45 degrees C. Their K-M for d-luciferin and ATP were 2.7 mu M and 92 mu M (D. nubilus), 3.7 mu M and 49 mu M (D. pectinealis), 3.5 mu M and 46 mu M (Diaphanes sp2). Phylogenetic analyses support that D. nubilus is sister to D. pectinealis with Diaphanes sp2 at their base, which further cluster with Pyrocoelia. All combined data indicate that sympatric Diaphanes species have similar luciferase characteristics, suggesting that other strategies (e.g., pheromone, active time, etc.) may be adopted to recognize mates. Our data provide new insights into Diaphanes luciferases and their evolution. Graphic abstract
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13765
专题	科研部门_遗传多样性基因组学研究组（张国捷）
推荐引用方式 GB/T 7714	He, JW,Liu, GC,Dong, PX,et al. Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes)[J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,2021,20(8):1053-1067.
APA	He, JW.,Liu, GC.,Dong, PX.,Dong, ZW.,Zhao, RP.,...&Li, XY.(2021).Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes).PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,20(8),1053-1067.
MLA	He, JW,et al."Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes)".PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 20.8(2021):1053-1067.

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