Modification of N-terminal alpha-amine of proteins via biomimetic ortho-quinone-mediated oxidation

	Modification of N-terminal alpha-amine of proteins via biomimetic ortho-quinone-mediated oxidation
	Wang, SY; Zhou, QQ; Chen, XP; Luo, RH; Li, YX; Liu, XL; Yang, LM; Zheng, YT; Wang, P
	2021
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	12 期号:1
摘要	Naturally abundant quinones are important molecules, which play essential roles in various biological processes due to their reduction potential. In contrast to their universality, the investigation of reactions between quinones and proteins remains sparse. Herein, we report the development of a convenient strategy to protein modification via a biomimetic quinone-mediated oxidation at the N-terminus. By exploiting unique reactivity of an ortho-quinone reagent, the alpha -amine of protein N-terminus is oxidized to generate aldo or keto handle for orthogonal conjugation. The applications have been demonstrated using a range of proteins, including myoglobin, ubiquitin and small ubiquitin-related modifier 2 (SUMO2). The effect of this method is further highlighted via the preparation of a series of 17 macrophage inflammatory protein 1 beta (MIP-1 beta) analogs, followed by preliminary anti-HIV activity and cell viability assays, respectively. This method offers an efficient and complementary approach to existing strategies for N-terminal modification of proteins. Methods for selective modification of the N-terminus of proteins are of high interest, but mostly require specific amino acid residues. Here, the authors report a selective and fast method for N-terminal modification of proteins based on quinone-mediated oxidation of the alpha-amine to aldehyde or ketone, and apply it to diverse proteins.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13802
专题	科研部门_分子免疫药理学（郑永唐）
推荐引用方式 GB/T 7714	Wang, SY,Zhou, QQ,Chen, XP,et al. Modification of N-terminal alpha-amine of proteins via biomimetic ortho-quinone-mediated oxidation[J]. NATURE COMMUNICATIONS,2021,12(1).
APA	Wang, SY.,Zhou, QQ.,Chen, XP.,Luo, RH.,Li, YX.,...&Wang, P.(2021).Modification of N-terminal alpha-amine of proteins via biomimetic ortho-quinone-mediated oxidation.NATURE COMMUNICATIONS,12(1).
MLA	Wang, SY,et al."Modification of N-terminal alpha-amine of proteins via biomimetic ortho-quinone-mediated oxidation".NATURE COMMUNICATIONS 12.1(2021).

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