| HIV-1 but not SIVmac239 induces higher interferon-alpha antiviral state in chronic infected northern pig-tailed macaques (Macaca leonina) |
| He, WQ; He, XY; Lu, Y; Zhang, S; Zhang, MX; Zheng, YT; Pang, W
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| 2022
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| 发表期刊 | MICROBES AND INFECTION
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| ISSN | 1286-4579
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| 卷号 | 24期号:5 |
| 摘要 | Studies have shown that interferon (IFN)-alpha has an inhibitory effect on human immunodeficiency virus type 1 (HIV-1) replication in the acute infection stage, but its role in chronic infection is still unclear. We previously established a nonpathogenic HIV-1 and pathogenic simian immunodeficiency virus (SIV) model in northern pig-tailed macaques (NPMs, Macaca leonina). In the current study, we detected viral RNA and DNA in various tissues (axillary lymph nodes (LNs), inguinal LNs, and spleen) in HIV-1(NL4-3)- and SIVmac239-infected NPM during the chronic stage of infection. Results indicated that the levels of viral DNA and RNA were higher in the tested tissues (LNs and spleen) of the SIVmac239-infected NPMs than in the HIV-1(NL4-3) infected NPMs. Furthermore, IFN-alpha expression was higher in the HIV-infected tissues than in the SIV-infected controls. The HIV restriction factors induced by IFN-alpha (i.e., tetherin and MX2), as well as inflammatory factors IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin 6 (IL-6), were analyzed using real-time polymerase chain reaction (PCR) and immunofluorescence staining assays. Results showed that their expression levels were much higher in the HIV-infected tissues than in the SIV-infected controls. These findings were confirmed by in vitro experiments on healthy NPM peripheral blood mononuclear cells infected with HIV-1(NL4-3), which showed lower viral replication, higher IFN-alpha expression, and an antiviral status. This study demonstrated that HIV-1 infection, but not SIVmac239 infection, in NPMs caused higher expression of IFN-alpha and induced a higher antiviral status. This may be one of the reasons why HIV-1 cannot replicate at a high level or develop into AIDS in NPMs. (C) 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
| 收录类别 | sci
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13812
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| 专题 | 科研部门_分子免疫药理学（郑永唐）
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推荐引用方式
GB/T 7714 |
He, WQ,He, XY,Lu, Y,et al. HIV-1 but not SIVmac239 induces higher interferon-alpha antiviral state in chronic infected northern pig-tailed macaques (Macaca leonina)[J]. MICROBES AND INFECTION,2022,24(5).
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| APA |
He, WQ.,He, XY.,Lu, Y.,Zhang, S.,Zhang, MX.,...&Pang, W.(2022).HIV-1 but not SIVmac239 induces higher interferon-alpha antiviral state in chronic infected northern pig-tailed macaques (Macaca leonina).MICROBES AND INFECTION,24(5).
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| MLA |
He, WQ,et al."HIV-1 but not SIVmac239 induces higher interferon-alpha antiviral state in chronic infected northern pig-tailed macaques (Macaca leonina)".MICROBES AND INFECTION 24.5(2022).
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