| Lyophyllin, a Mushroom Protein from the Peptidase M35 Superfamily Is an RNA N-Glycosidase |
| Lu, JQ; Shi, WW; Xiao, MJ; Tang, YS; Zheng, YT; Shaw, PC
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| 2021
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| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 卷号 | 22期号:21 |
| 摘要 | Ribosome-inactivating proteins (RIPs) hydrolyze the N-glycosidic bond and depurinate a specific adenine residue (A-4324 in rat 28S ribosomal RNA, rRNA) in the conserved alpha-sarcin/ricin loop (alpha-SRL) of rRNA. In this study, we have purified and characterized lyophyllin, an unconventional RIP from Lyophyllum shimeji, an edible mushroom. The protein resembles peptidase M35 domain of peptidyl-Lys metalloendopeptidases. Nevertheless, protein either from the mushroom or in recombinant form possessed N-glycosidase and protein synthesis inhibitory activities. A homology model of lyophyllin was constructed. It was found that the zinc binding pocket of this protein resembles the catalytic cleft of a classical RIP, with key amino acids that interact with the adenine substrate in the appropriate positions. Mutational studies showed that E122 may play a role in stabilizing the positively charged oxocarbenium ion and H121 for protonating N-3 of adenine. The tyrosine residues Y137 and Y104 may be used for stacking the target adenine ring. This work first shows a protein in the peptidase M35 superfamily based on conserved domain search possessing N-glycosidase activity. |
| 收录类别 | sci
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13843
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| 专题 | 科研部门_分子免疫药理学（郑永唐）
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推荐引用方式
GB/T 7714 |
Lu, JQ,Shi, WW,Xiao, MJ,et al. Lyophyllin, a Mushroom Protein from the Peptidase M35 Superfamily Is an RNA N-Glycosidase[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(21).
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| APA |
Lu, JQ,Shi, WW,Xiao, MJ,Tang, YS,Zheng, YT,&Shaw, PC.(2021).Lyophyllin, a Mushroom Protein from the Peptidase M35 Superfamily Is an RNA N-Glycosidase.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(21).
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| MLA |
Lu, JQ,et al."Lyophyllin, a Mushroom Protein from the Peptidase M35 Superfamily Is an RNA N-Glycosidase".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.21(2021).
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