| Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2 |
| Xia, BQ; Pan, XY; Luo, RH; Shen, XR; Li, SQ; Wang, Y; Zuo, XL; Wu, Y; Guo, YQ; Xiao, GF; Li, QG; Long, XY; He, XY; Zheng, HY; Lu, Y; Pang, W; Zheng, YT; Li, J; Zhang, LK ; Gao, ZB
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| 2023
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| 发表期刊 | CELL DISCOVERY
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| 卷号 | 9期号:1 |
| 摘要 | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic. Antibody resistance dampens neutralizing antibody therapy and threatens current global Coronavirus (COVID-19) vaccine campaigns. In addition to the emergence of resistant SARS-CoV-2 variants, little is known about how SARS-CoV-2 evades antibodies. Here, we report a novel mechanism of extracellular vesicle (EV)-mediated cell-to-cell transmission of SARS-CoV-2, which facilitates SARS-CoV-2 to escape from neutralizing antibodies. These EVs, initially observed in SARS-CoV-2 envelope protein-expressing cells, are secreted by various SARS-CoV-2-infected cells, including Vero E6, Calu-3, and HPAEpiC cells, undergoing infection-induced pyroptosis. Various SARS-CoV-2-infected cells produce similar EVs characterized by extra-large sizes (1.6-9.5 mu m in diameter, average diameter > 4.2 mu m) much larger than previously reported virus-generated vesicles. Transmission electron microscopy analysis and plaque assay reveal that these SARS-CoV-2-induced EVs contain large amounts of live virus particles. In particular, the vesicle-cloaked SARS-CoV-2 virus is resistant to neutralizing antibodies and able to reinfect naive cells independent of the reported receptors and cofactors. Consistently, the constructed 3D images show that intact EVs could be taken up by recipient cells directly, supporting vesicle-mediated cell-to-cell transmission of SARS-CoV-2. Our findings reveal a novel mechanism of receptor-independent SARS-CoV-2 infection via cell-to-cell transmission, provide new insights into antibody resistance of SARS-CoV-2 and suggest potential targets for future antiviral therapeutics. |
| 收录类别 | SCI
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13967
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| 专题 | 科研部门_分子免疫药理学（郑永唐）
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推荐引用方式
GB/T 7714 |
Xia, BQ,Pan, XY,Luo, RH,et al. Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2[J]. CELL DISCOVERY,2023,9(1).
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| APA |
Xia, BQ.,Pan, XY.,Luo, RH.,Shen, XR.,Li, SQ.,...&Gao, ZB.(2023).Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2.CELL DISCOVERY,9(1).
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| MLA |
Xia, BQ,et al."Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2".CELL DISCOVERY 9.1(2023).
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