| Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation |
| Yang, W; Liu, LB; Liu, FL; Wu, YH; Zhen, ZD; Fan, DY; Sheng, ZY; Song, ZR; Chang, JT; Zheng, YT; An, J; Wang, PG
|
| 2023
|
| 发表期刊 | NATURE COMMUNICATIONS
 |
| 卷号 | 14期号:1 |
| 摘要 | Zika virus (ZIKV) is a potential threat to male reproductive health but the mechanisms underlying its influence on testes during ZIKV infection remain obscure. To address this question, we perform single-cell RNA sequencing using testes from ZIKV-infected mice. The results reveal the fragility of spermatogenic cells, especially spermatogonia, to ZIKV infection and showthat the genes of the complement system are significantly upregulated mainly in infiltrated S100A4 + monocytes/macrophages. Complement activation and its contribution to testicular damage are validated by ELISA, RT.qPCR and IFA and further verify in ZIKV-infected northern pigtailed macaques by RNA genome sequencing and IFA, suggesting that thismight be the common response to ZIKV infection in primates. On this basis, we test the complement inhibitor C1INH and S100A4 inhibitors sulindac and niclosamide for their effects on testis protection. C1INH alleviates the pathological change in the testis but deteriorates ZIKV infection in general. In contrast, niclosamide effectively reduces S100A4 + monocyte/macrophage infiltration, inhibits complement activation, alleviates testicular damage, and rescues the fertility of male mice from ZIKV infection. This discovery therefore encourages male reproductive health protection during the next ZIKV epidemic. |
| 收录类别 | SCI
|
| 语种 | 英语
|
| 文献类型 | 期刊论文
|
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13975
|
| 专题 | 科研部门_分子免疫药理学（郑永唐）
|
推荐引用方式
GB/T 7714 |
Yang, W,Liu, LB,Liu, FL,et al. Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation[J]. NATURE COMMUNICATIONS,2023,14(1).
|
| APA |
Yang, W.,Liu, LB.,Liu, FL.,Wu, YH.,Zhen, ZD.,...&Wang, PG.(2023).Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation.NATURE COMMUNICATIONS,14(1).
|
| MLA |
Yang, W,et al."Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation".NATURE COMMUNICATIONS 14.1(2023).
|
修改评论