Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis

	Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis
	Zhang, WD; Tang, M; Wang, L; Zhou, H; Gao, J; Chen, ZL; Zhao, B; Zheng, P
	2023
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	9 期号:4
摘要	Replication stress is a major source of endogenous DNA damage. Despite the identification of numerous pro-teins on replication forks to modulate fork or replication machinery activities, it remains unexplored whether noncoding RNAs can localize on stalled forks and play critical regulatory roles. Here, we identify an uncharac-terized long noncoding RNA NONMMUT028956 (Lnc956 for short) predominantly expressed in mouse embry-onic stem cells. Lnc956 is accumulated on replication forks to prevent fork collapse and preserve genomic stability and is essential for mouse embryogenesis. Mechanistically, it drives assembly of the Lnc956-TRIM28-HSP90B1 complex on stalled forks in an interdependent manner downstream of ataxia telangiectasia and Rad3-related (ATR) signaling. Lnc956-TRIM28-HSP90B1 complex physically associates with minichromosome mainte-nance proteins 2 (MCM2) to minichromosome maintenance proteins 7 (MCM7) hexamer via TRIM28 and directly regulates the CDC45-MCM-GINS (CMG) helicase retention on chromatin. The regulation of Lnc956-TRIM28-HSP90B1 on CMG retention is mediated by HSP90B1's chaperoning function. These findings reveal a player that actively regulates replisome retention to prevent fork collapse.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13984
专题	科研部门_哺乳动物胚胎发育（郑萍）
推荐引用方式 GB/T 7714	Zhang, WD,Tang, M,Wang, L,et al. Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis[J]. SCIENCE ADVANCES,2023,9(4).
APA	Zhang, WD.,Tang, M.,Wang, L.,Zhou, H.,Gao, J.,...&Zheng, P.(2023).Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis.SCIENCE ADVANCES,9(4).
MLA	Zhang, WD,et al."Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis".SCIENCE ADVANCES 9.4(2023).

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