Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells

	Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells
	Gong, DH; Wang, L; Zhou, H; Gao, J; Zhang, WD; Zheng, P
	2023
发表期刊	STEM CELL REPORTS
ISSN	2213-6711
卷号	18 期号:4 页码:952-968
摘要	Embryonic stem cells (ESCs) are superior to differentiated cells to maintain genome stability, but the underlying mechanisms remain largely elusive. R-loops are constantly formed during transcription and are inducers of DNA damage if not resolved. Here we report that mouse ESCs (mESCs) can efficiently prevent unscheduled R-loop formation, and a long noncoding RNA Lnc530 plays regulatory role. Lnc530 is expressed in mESCs and localizes on R-loops. Depletion of Lnc530 in mESCs causes R-loop accumulation and DNA damage, whereas forced expression of Lnc530 in differentiated cells suppresses the R-loop formation. Mechanistically, Lnc530 associates with DDX5 and TDP-43 in an inter-dependent manner on R-loops. Formation of Lnc530-DDX5-TDP-43 complex substantially increases the local protein levels of DDX5 and TDP-43, both of which play critical roles in R-loop regulation. This study uncovers an efficient strategy to prevent R-loop accumulation and preserve genomic stability in mESCs and possibly other stem cell types.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13985
专题	科研部门_哺乳动物胚胎发育（郑萍）
推荐引用方式 GB/T 7714	Gong, DH,Wang, L,Zhou, H,et al. Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells[J]. STEM CELL REPORTS,2023,18(4):952-968.
APA	Gong, DH,Wang, L,Zhou, H,Gao, J,Zhang, WD,&Zheng, P.(2023).Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells.STEM CELL REPORTS,18(4),952-968.
MLA	Gong, DH,et al."Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells".STEM CELL REPORTS 18.4(2023):952-968.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2024061466.pdf（6737KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文