Scrapie-associated polymorphisms of the prion protein gene (PRNP) in Nigerian native goats

	Scrapie-associated polymorphisms of the prion protein gene (PRNP) in Nigerian native goats
	Adeola, AC; Bello, SF; Abdussamad, AM; Mark, AI; Sanke, OJ; Onoja, AB; Nneji, LM; Abdullahi, N; Olaogun, SC; Rogo, LD; Mangbon, GF; Pedro, SL; Hiinan, MP; Mukhtar, MM; Ibrahim, J; Saidu, H; Dawuda, PM; Bala, RK; Abdullahi, HL; Salako, AE; Kdidi, S; Yahyaoui, MH; Yin, TT
	2023
发表期刊	GENE
ISSN	0378-1119
卷号	855
摘要	Scrapie is a fatal prion protein disease stiffly associated with single nucleotide polymorphism (SNPs) of the prion protein gene (PRNP). The prevalence of this deadly disease has been reported in small ruminants, including goats. The Nigerian goats are hardy, trypano-tolerant, and contribute to the protein intake of the increasing population. Although scrapie has been reported in Nigerian goats, there is no study on the polymorphism of the PRNP gene. Herein, we evaluated the genetic and allele distributions of PRNP polymorphism in 132 Nigerian goats and compared them with publicly available studies on scrapie-affected goats. We utilized Polyphen-2, PROVEAN and AMYCO programs to examine structural variations produced by the non-synonymous SNPs. Our study revealed 29 SNPs in Nigerian goats, of which 14 were non-synonymous, and 23 were novel. There were significant differences (P < 0.001) in the allele frequencies of PRNP codons 139, 146, 154 and 193 in Nigerian goats compared with scrapie-affected goats, except for Northern Italian goats at codon 154. Based on the prediction by Polyphen-2, R139S and N146S were 'benign', R154H was 'probably damaging', and T193I was 'possibly damaging'. In contrast, PROVEAN predicted 'neutral' for all non-synonymous SNPs, while AMYCO showed a similar amyloid propensity of PRNP for resistant haplotype and two haplotypes of Nigerian goats. Our study is the first to investigate the polymorphism of scrapie-related genes in Nigerian goats.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13992
专题	科研部门_分子进化与基因组多样性（张亚平）
推荐引用方式 GB/T 7714	Adeola, AC,Bello, SF,Abdussamad, AM,et al. Scrapie-associated polymorphisms of the prion protein gene (PRNP) in Nigerian native goats[J]. GENE,2023,855.
APA	Adeola, AC.,Bello, SF.,Abdussamad, AM.,Mark, AI.,Sanke, OJ.,...&Yin, TT.(2023).Scrapie-associated polymorphisms of the prion protein gene (PRNP) in Nigerian native goats.GENE,855.
MLA	Adeola, AC,et al."Scrapie-associated polymorphisms of the prion protein gene (PRNP) in Nigerian native goats".GENE 855(2023).

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