Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor

	Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor
	Yousefi, M; Lee, WS; Chan, WOY; He, W; Mah, MG; Yong, CL; Deerain, JM; Wang, LJ; Arcinas, C; Yan, BG; Tan, DW; Sia, WR; Gamage, AM; Yang, JX; Hsu, ACY; Li, S; Linster, M; Yang, XL; Ghosh, S; Anderson, DE; Smith, GJD; Tan, CW; Wang, LF; Ooi, YS
	2023
发表期刊	EMERGING MICROBES & INFECTIONS
卷号	12 期号:2
摘要	The emergence of novel betacoronaviruses has posed significant financial and human health burdens, necessitating the development of appropriate tools to combat future outbreaks. In this study, we have characterized a human cell line, IGROV-1, as a robust tool to detect, propagate, and titrate betacoronaviruses SARS-CoV-2 and HCoV-OC43. IGROV-1 cells can be used for serological assays, antiviral drug testing, and isolating SARS-CoV-2 variants from patient samples. Using time-course transcriptomics, we confirmed that IGROV-1 cells exhibit a robust innate immune response upon SARS-CoV-2 infection, recapitulating the response previously observed in primary human nasal epithelial cells. We performed genome-wide CRISPR knockout genetic screens in IGROV-1 cells and identified Aryl hydrocarbon receptor (AHR) as a critical host dependency factor for both SARS-CoV-2 and HCoV-OC43. Using DiMNF, a small molecule inhibitor of AHR, we observed that the drug selectively inhibits HCoV-OC43 infection but not SARS-CoV-2. Transcriptomic analysis in primary normal human bronchial epithelial cells revealed that DiMNF blocks HCoV-OC43 infection via basal activation of innate immune responses. Our findings highlight the potential of IGROV-1 cells as a valuable diagnostic and research tool to combat betacoronavirus diseases.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14038
专题	科研部门_疫源动物病毒（杨兴娄）
推荐引用方式 GB/T 7714	Yousefi, M,Lee, WS,Chan, WOY,et al. Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor[J]. EMERGING MICROBES & INFECTIONS,2023,12(2).
APA	Yousefi, M.,Lee, WS.,Chan, WOY.,He, W.,Mah, MG.,...&Ooi, YS.(2023).Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor.EMERGING MICROBES & INFECTIONS,12(2).
MLA	Yousefi, M,et al."Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor".EMERGING MICROBES & INFECTIONS 12.2(2023).

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2024053182.pdf（5064KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文